Rabbit anti-Human, Rat UBE2E3 Polyclonal Antibody | anti-UBE2E3 antibody

UBE2E3 Polyclonal Antibody

Cat. Num. AAA3006866

• Gene Names: UBE2E3; UBCH9; UbcM2
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UBE2E3; Polyclonal Antibody; UBE2E3 Polyclonal Antibody; Ubiquitin-conjugating enzyme E2 E3; UbcH9; Ubiquitin carrier protein E3; Ubiquitin-conjugating enzyme E2-23 kDa; Ubiquitin-protein ligase E3; UBCE4; UBCH9; anti-UBE2E3 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UBE2E3 polyclonal antibody detects endogenous levels of UBE2E3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 207

• Applicable Applications for anti-UBE2E3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human UBE2E3
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of UBE2E3 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:PC12 whole cell lysate)

Related Product Information for anti-UBE2E3 antibody

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.The first step requires the ATP-dependent activation of the Ub C-terminusand the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. A wide range of enzymes facilitate in the proteolytic Ub pathway including UBE2E3, also designated UBCH9, which catalyzes the covalent attachment of ubiquitin to other proteins and is involved in the regulation of transepithelial sodium transport in renal cells. UBE2E3 may also be involved in cell growth arrest. The UBE2E3 protein shuttles between the cytoplasm and nucleus in a IPO11-dependent manner. It is ubiquitously expressed at low levels and is highly expressed in skeletal muscle.

NCBI and Uniprot Product Information

• NCBI GI #: 514052664
• NCBI GeneID: 10477
• NCBI Accession #: NP_001265483.1
• NCBI GenBank Nucleotide #: NP_001265483.1
• UniProt Accession #: Q969T4
• Molecular Weight: ~ 23kDa
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 E3
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 E3
• NCBI Official Symbol: UBE2E3
• NCBI Official Synonym Symbols: UBCH9; UbcM2
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 E3
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 E3
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2E3
• UniProt Synonym Gene Names: UBCE4; UBCH9
• UniProt Entry Name: UB2E3_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

UBE2E3: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 2q32.1

Molecular Function: protein binding; ubiquitin-protein ligase activity