Western Blot (WB)
(Western blot analysis of GBE1 Antibody (Center) in HL-60 cell line lysates (35ug/lane). GBE1 (arrow) was detected using the purified Pab;)
Rabbit anti-Human, Mouse GBE1 Polyclonal Antibody
GBE1, ID (GBE1, 1,4-alpha-glucan-branching enzyme, Brancher enzyme, Glycogen-branching enzyme)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
GBE1; Polyclonal Antibody; ID (GBE1; 1; 4-alpha-glucan-branching enzyme; Brancher enzyme; Glycogen-branching enzyme); Anti -GBE1; anti-GBE1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-GBE1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunofluorescence: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunofluorescence: 1:10-50
Immunogen
GBE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 534~564 amino acids from the Central region of human GBE1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of GBE1 Antibody (Center) in HL-60 cell line lysates (35ug/lane). GBE1 (arrow) was detected using the purified Pab;)
Western Blot (WB)
(Western blot analysis of GBE1 Antibody (Center) in HL-60 cell line lysates (35ug/lane). GBE1 (arrow) was detected using the purified Pab;)
Western Blot (WB)
(Western blot analysis of GBE1 Antibody (Center) in mouse liver tissue lysates (35ug/lane).GBE1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of GBE1 Antibody (Center) in mouse liver tissue lysates (35ug/lane).GBE1 (arrow) was detected using the purified Pab.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of GBE1 Antibody (Center) with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of GBE1 Antibody (Center) with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-GBE1 antibody
GBE1 is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease).
Product Categories/Family for anti-GBE1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The GBE1 (Catalog #AAA6000399) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GBE1, ID (GBE1, 1,4-alpha-glucan-branching enzyme, Brancher enzyme, Glycogen-branching enzyme) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GBE1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in Western Blot, Immunofluorescence, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunofluorescence: 1:10-50. Researchers should empirically determine the suitability of the GBE1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GBE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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