Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: |Representative lot data. |Confocal fluorescent analysis of A431 and HeLa cells using Ubch9 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye- Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus and cytoplasm.)
Mouse anti-Human, Mouse UBCH9 Monoclonal Antibody | anti-UBE2E3 antibody
UBCH9 (Ubiquitin-conjugating Enzyme E2 E3, Ubiquitin-protein Ligase E3, Ubiquitin Carrier Protein E3, Ubiquitin-conjugating Enzyme E2-23kD, UbcH9, UBE2E3, UBCE4)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Western Blot: 0.5ug/ml
Immunocytochemistry: 1ug/ml
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: |Representative lot data. |Confocal fluorescent analysis of A431 and HeLa cells using Ubch9 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye- Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus and cytoplasm.)
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: |Representative lot data. |Confocal fluorescent analysis of A431 and HeLa cells using Ubch9 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye- Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus and cytoplasm.)
Western Blot (WB)
(Western Blotting Analysis: |Representative lot data. |NIH/3T3 cell lysate was probed with Ubch9 (0.5ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.|Arrow indicates Ubch9 (~23kD). An uncharacterized band at a molecular weight of ~19kD may be observed in some cell lysates.)
Western Blot (WB)
(Western Blotting Analysis: |Representative lot data. |NIH/3T3 cell lysate was probed with Ubch9 (0.5ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.|Arrow indicates Ubch9 (~23kD). An uncharacterized band at a molecular weight of ~19kD may be observed in some cell lysates.)
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2013]
Uniprot Description
Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest. Ref.1 Ref.11
Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway: Protein modification; protein ubiquitination.
Subunit structure: The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus
By similarity. Interacts with NEDD4L.
Subcellular location: Nucleus. Cytoplasm. Note: Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner. Ref.9
Tissue specificity: Ubiquitously expressed at low levels. Highly expressed in skeletal muscle. Ref.1
Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
Research Articles on UBE2E3
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Product Notes
The UBE2E3 ube2e3 (Catalog #AAA647628) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The UBCH9 (Ubiquitin-conjugating Enzyme E2 E3, Ubiquitin-protein Ligase E3, Ubiquitin Carrier Protein E3, Ubiquitin-conjugating Enzyme E2-23kD, UbcH9, UBE2E3, UBCE4) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UBCH9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in Western Blot and Immunocytochemistry. Dilution: Western Blot: 0.5ug/ml Immunocytochemistry: 1ug/ml. Researchers should empirically determine the suitability of the UBE2E3 ube2e3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBCH9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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