Mouse anti-Human CFLAR / FLIP Monoclonal Antibody | anti-CFLAR antibody

Mouse Monoclonal [clone 2F12] (IgG2a,k) to Human CFLAR / FLIP

Cat. Num. AAA246554

• Gene Names: CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: CFLAR / FLIP; Monoclonal Antibody; Mouse Monoclonal [clone 2F12] (IgG2a; k) to Human CFLAR / FLIP; Anti-CFLAR / FLIP Antibody (clone 2F12) IHC-plus; CFLAR; C-FLIPR; C-FLIPS; C-FLIP; CASP8AP1; Cflip; CLARP; CASH; Caspase homolog; Caspase-eight-related protein; Flice inhibitory protein; I-FLICE; Inhibitor of FLICE; FLAME; FLAME1; MRIT; Usurpin beta; C-FLIPL; Casper; FLAME-1; FLIP; Usurpin; Human CFLAR; anti-CFLAR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2F12
• Specificity: Human CFLAR
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.47 mg/ml (varies by lot)
• Sequence Length: 480

• Applicable Applications for anti-CFLAR antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (10 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: CFLAR (AAH01602, 178 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: CFLAR / FLIP antibody was raised against cFLAR (AAH01602, 178 a.a. ~ 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-CFLAR / FLIP antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CFLAR / FLIP antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to CFLAR on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(CFLAR monoclonal antibody, clone 2F12 Western blot of CFLAR expression in IMR-32.)

ELISA (EIA)

(Detection limit for recombinant GST tagged CFLAR is approximately 0.3 ng/ml as a capture antibody.)

Related Product Information for anti-CFLAR antibody

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively.

Product Categories/Family for anti-CFLAR antibody

Family: Apoptosis; Subfamily: Cysteine C14

NCBI and Uniprot Product Information

• NCBI GI #: 187608571
• NCBI GeneID: 8837
• NCBI Accession #: NP_003870.4
• NCBI GenBank Nucleotide #: NM_003879.5
• UniProt Accession #: O15519; O14673; O14674; O14675; O15137; O15138; O15356; O15510; O43618; O43619; B4DJE0; B7Z9F9
• Molecular Weight: 44,112 Da
• NCBI Official Full Name: CASP8 and FADD-like apoptosis regulator isoform 1
• NCBI Official Synonym Full Names: CASP8 and FADD-like apoptosis regulator
• NCBI Official Symbol: CFLAR
• NCBI Official Synonym Symbols: CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• NCBI Protein Information: CASP8 and FADD-like apoptosis regulator; usurpin beta; caspase homolog; inhibitor of FLICE; caspase-eight-related protein; MACH-related inducer of toxicity; FADD-like antiapoptotic molecule 1; caspase-related inducer of apoptosis; cellular FLICE-like inhibitory protein; caspase-like apoptosis regulatory protein
• UniProt Protein Name: CASP8 and FADD-like apoptosis regulator
• Protein Family: CASP8 and FADD-like apoptosis regulator
• UniProt Gene Name: CFLAR
• UniProt Synonym Gene Names: CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT
• UniProt Entry Name: CFLAR_HUMAN

NCBI Description

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]

Uniprot Description

CFLAR: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. TNFRSF6 stimulation triggers recruitment to the death- inducing signaling complex (DISC) formed by TNFRSF6, FADD and caspase-8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with caspase-10, caspase-3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with HBV protein X. Repressed by IL2/interleukin-2 after TCR stimulation, during progression to the S phase of the cell cycle. Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. Belongs to the peptidase C14A family. 14 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: cytoplasm; CD95 death-inducing signaling complex; cytosol; lipid raft

Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; death receptor binding; enzyme activator activity; protein complex binding

Biological Process: caspase activation; skeletal muscle development; positive regulation of I-kappaB kinase/NF-kappaB cascade; skeletal muscle atrophy; skeletal myofibril assembly; viral reproduction; apoptosis; skeletal muscle regeneration; regulation of satellite cell proliferation; proteolysis; activation of NF-kappaB transcription factor; negative regulation of apoptosis