Testing Data #1
(Western blot analysis of total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K0) and NIH/3T3 cells probed with Rabbit anti FLIP gamma/delta (MBS223330))
Rabbit anti-Mouse FLIP GAMMA/DELTA Polyclonal Antibody | anti-FLIP GAMMA/DELTA antibody
RABBIT ANTI FLIP GAMMA/DELTA (C-TERMINAL)
FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1.
FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. This item recognizes human and murine delta and gamma variants but not the alpha or beta, variants.
FLIP is widely expressed in human tissues and a diverse range of cell lines.
Purified IgG - liquid
Immunohistology - Frozen: Maximum Dilution: 2ug/ml
Western Blotting: Maximum Dilution: 1/1000
Buffer Solution
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Western blot analysis of total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K0) and NIH/3T3 cells probed with Rabbit anti FLIP gamma/delta (MBS223330))
Testing Data #1
(Western blot analysis of total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K0) and NIH/3T3 cells probed with Rabbit anti FLIP gamma/delta (MBS223330))
Testing Data #2
(Immunocytochemical staining of HeLa cells with Rabbit anti FLIP gamma/ delta (MBS223330))
Testing Data #2
(Immunocytochemical staining of HeLa cells with Rabbit anti FLIP gamma/ delta (MBS223330))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
Uniprot Description
CFLAR: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. TNFRSF6 stimulation triggers recruitment to the death- inducing signaling complex (DISC) formed by TNFRSF6, FADD and caspase-8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with caspase-10, caspase-3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with HBV protein X. Repressed by IL2/interleukin-2 after TCR stimulation, during progression to the S phase of the cell cycle. Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. Belongs to the peptidase C14A family. 14 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 2q33-q34
Cellular Component: cytoplasm; CD95 death-inducing signaling complex; cytosol; lipid raft
Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; enzyme activator activity; death receptor binding; protein complex binding
Biological Process: caspase activation; skeletal muscle development; skeletal myofibril assembly; skeletal muscle atrophy; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; skeletal muscle regeneration; regulation of satellite cell proliferation; proteolysis; activation of NF-kappaB transcription factor; negative regulation of apoptosis
Research Articles on FLIP GAMMA/DELTA
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Product Notes
The FLIP GAMMA/DELTA cflar (Catalog #AAA223330) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI FLIP GAMMA/DELTA (C-TERMINAL) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FLIP GAMMA/DELTA can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Western Blot (WB). Western Blot: This item detects bands of approximately 35kDa and 25kDa in Jurkat cell lysates. Immunohistology - Frozen: Maximum Dilution: 2ug/ml Western Blotting: Maximum Dilution: 1/1000. Researchers should empirically determine the suitability of the FLIP GAMMA/DELTA cflar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FLIP GAMMA/DELTA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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