Human Androgen Receptor (AR) ELISA Kit | AR elisa kit

Human Androgen Receptor (AR) ELISA Kit

Cat. Num. AAA2703078

• Gene Names: AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• Reactivity: Human
• Synonyms: Androgen Receptor (AR); Human Androgen Receptor (AR) ELISA Kit; AIS; DHTR; HUMARA; KD; NR3C4; SBMA; SMAX1; TFM; Dihydrotestosterone receptor; Nuclear Receptor Subfamily 3; Group C; Member 4; Testicular Feminization; Kennedy Disease; AR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Androgen Receptor (AR).; No significant cross-reactivity or interference between Androgen Receptor (AR) and analogues was observed.
• Sequence Length: 648

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Androgen Receptor (AR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Androgen Receptor (AR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Androgen Receptor (AR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Androgen Receptor (AR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Androgen Receptor (AR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Androgen Receptor (AR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 178882
• NCBI GeneID: 367
• UniProt Accession #: P10275; Q9UD95; A0A0B4J1T2; A2RUN2; B1AKD7; C0JKD3; C0JKD4; E7EVX6
• Molecular Weight: 67,659 Da
• NCBI Official Full Name: androgen receptor
• NCBI Official Synonym Full Names: androgen receptor
• NCBI Official Symbol: AR
• NCBI Official Synonym Symbols: KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• NCBI Protein Information: androgen receptor
• UniProt Protein Name: Androgen receptor
• Protein Family: Allatostatin
• UniProt Gene Name: AR
• UniProt Synonym Gene Names: DHTR; NR3C4

NCBI Description

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

Uniprot Description

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.