Mouse anti-Human CD96 Monoclonal Antibody | anti-CD96 antibody

Anti-Hu CD96 Purified

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified by protein-A affinity chromatography
• Synonyms: CD96; Monoclonal Antibody; Anti-Hu CD96 Purified; TACTILE; MGC22596; CD96 molecule; anti-CD96 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: NK92.39
• Specificity: The mouse monoclonal antibody NK92.39 recognizes an extracellular epitope of CD96, a transmembrane glycoprotein expressed at low levels on resting, and at high levels on activated T and NK cells, as well as on many T cell leukemias.
• Purity/Purification: Purified by protein-A affinity chromatography
• Form/Format: Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD96 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: Recommended dilution: 1-12ug/ml
• Immunogen: human NK92 cell line
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD96 (NK92.39) Purified antibody (concentration in sample 5 ug/ml, GAM APC).)

Flow Cytometry (FC/FACS)

(Separation of human lymphocytes stained anti-human CD96 (NK92.39) purified antibody (concentration in sample 5 ug/ml, GAM-APC, red-filled) from human lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).)

Related Product Information for anti-CD96 antibody

CD96 is a 160-240kDa type I transmembrane glycoprotein with intracellular ITIM-like domain, however, without observed inhibitory Functional Application (FUNC)tion. This protein is involved in adhesive interactions of T and NK cells during the late phase of the immune response, and are expressed above all upon their activation. Strong interaction between CD96 and CD155 (poliovirus receptor) stimulates cytotoxicity of activated NK cells, which may play a role in NK cell recognition and killing of CD155-expressing tumor cells. CD96 is expressed on approximately 80% of T cell leukemias and 30% of acute myeloid leukemias and is being used for their immunophenotyping.

References

*Eriksson EM, Keh CE, Deeks SG, Martin JN, Hecht FM, Nixon DF: Differential expression of CD96 surface molecule represents CD8? T cells with dissimilar effector Functional Application (FUNC)tion during HIV-1 infection. PLoS One. 2012;7(12):e51696., URL: https://www.ncbi.nlm.nih.gov/pubmed/23272144,*Fuchs A, Cella M, Giurisato E, Shaw AS, Colonna M: Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155). J Immunol. 2004 Apr 1;172(7):3994-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=cd96+(tactile)+promotes+NK+cell

NCBI and Uniprot Product Information

• NCBI GeneID: 10225
• UniProt Accession #: Q8WUE2
• Protein Family: T-cell surface protein tactile