Mouse anti-Human CD155 Monoclonal Antibody | anti-CD155 antibody

Anti-Hu CD155 Purified

• Gene Names: PVR; PVS; HVED; CD155; NECL5; TAGE4; Necl-5
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Purified by protein-A affinity chromatography
• Synonyms: CD155; Monoclonal Antibody; Anti-Hu CD155 Purified; PVR; HVED; NECL5; PVS; TAGE4; CMVR; PVR cell adhesion molecule; anti-CD155 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: SKII.4
• Specificity: The mouse monoclonal antibody SKII.4 recognizes an extracellular epitope of CD155, a transmembrane glycoprotein, known as poliovirus and cytomegalovirus receptor.
• Purity/Purification: Purified by protein-A affinity chromatography
• Form/Format: Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD155 antibody: Western Blot (WB), Flow Cytometry (FC/FACS) (QC tested), Immunoprecipitation (IP)
• Application Notes: FC/FACS: Recommended dilution: 2-6ug/ml
• Immunogen: human neuroblastoma SK-L cells
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD155 (SKII.4) Purified antibody (concentration in sample 1,6 ug/ml, GAM APC).)

Flow Cytometry (FC/FACS)

(Separation of U937 stained using anti-human CD155 (SKII.4) purified antibody (concentration in sample 1,6 ug/ml, GAM-APC, red-filled) from U937 unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).)

Related Product Information for anti-CD155 antibody

CD155 is a type I transmembrane glycoprotein of the nectin family, which serves as an adhesion molecule involved in intercellular and cell-extracellular matrix interactions. CD155 (nectin-like 5) heterodimerizes with CD113 (nectin-3) and this complex binds to CD51/CD61 complex (integrin alphaVbeta3), and to vitronectin. CD155 is also thought to regulate CD44 binding to hyaluronan, and serves as a receptor for poliovirus and cytomegalovirus. It is expressed on endothelial cells, epithelia, central nervous system, and monocytes.

References

*Boles KS, Vermi W, Facchetti F, Fuchs A, Wilson TJ, Diacovo TG, Cella M, Colonna M: A novel molecular interaction for the adhesion of follicular CD4 T cells to follicular DC. Eur J Immunol. 2009 Mar;39(3):695-703., URL: https://www.ncbi.nlm.nih.gov/pubmed/19197944,*Jin HS, Choi DS, Ko M, Kim D, Lee DH, Lee S, Lee AY, Kang SG, Kim SH, Jung Y, Jeong Y, Chung JJ, Park Y: Extracellular pH modulating injectable gel for enhancing immune checkpoint inhibitor therapy. J Control Release. 2019 Oct 25;315:65-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/31669264,*Fionda C, Abruzzese MP, Zingoni A, Soriani A, Ricci B, Molfetta R, Paolini R, Santoni A, Cippitelli M: Nitric oxide donors increase PVR/CD155 DNAM-1 ligand expression in multiple myeloma cells: role of DNA damage response activation. BMC Cancer. 2015 Jan 22;15:17., URL: https://www.ncbi.nlm.nih.gov/pubmed/25609078,*Davis ZB, Sowrirajan B, Cogswell A, Ward JP, Planelles V, Barker E: CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells. AIDS Res Hum Retroviruses. 2017 Feb;33(2):93-100., URL: https://www.ncbi.nlm.nih.gov/pubmed/27296670,*Lozano E, Dominguez-Villar M, Kuchroo V, Hafler DA: The TIGIT/CD226 axis regulates human T cell Functional Application (FUNC)tion. J Immunol. 2012 Apr 15;188(8):3869-75, URL: https://www.ncbi.nlm.nih.gov/pubmed/22427644,*Josefsson SE, Beiske K, Blaker YN, Førsund MS, Holte H, Østenstad B, Kimby E, Köksal H, Wälchli S, Bai B, Smeland EB, Levy R, Kolstad A, Huse K, Myklebust JH: TIGIT and PD-1 Mark Intratumoral T Cells with Reduced Effector Functional Application (FUNC)tion in B-cell Non-Hodgkin Lymphoma. Cancer Immunol Res. 2019 Mar;7(3):355-362., URL: https://www.ncbi.nlm.nih.gov/pubmed/30659053,*Fuchs A, Cella M, Giurisato E, Shaw AS, Colonna M: Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155). J Immunol. 2004 Apr 1;172(7):3994-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15034010

NCBI and Uniprot Product Information

• NCBI GI #: 609878250
• NCBI GeneID: 5817
• NCBI Accession #: NP_006496.4
• NCBI GenBank Nucleotide #: NM_006505.4
• UniProt Accession #: P15151; P15152; Q15267; Q15268; Q96BJ1; B4DTS9
• Molecular Weight: 45,303 Da
• NCBI Official Full Name: poliovirus receptor isoform alpha
• NCBI Official Synonym Full Names: poliovirus receptor
• NCBI Official Symbol: PVR
• NCBI Official Synonym Symbols: PVS; HVED; CD155; NECL5; TAGE4; Necl-5
• NCBI Protein Information: poliovirus receptor; nectin-like protein 5
• UniProt Protein Name: Poliovirus receptor
• UniProt Gene Name: PVR
• UniProt Synonym Gene Names: PVS; NECL-5
• UniProt Entry Name: PVR_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

PVR: Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. Belongs to the nectin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Immunoglobulin superfamily; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; cell surface; focal adhesion; cytoplasm; plasma membrane; integral to membrane

Molecular Function: viral receptor activity; protein binding; receptor activity; cell adhesion molecule binding

Biological Process: intercellular junction assembly and maintenance; regulation of immune response; cell migration; entry of virus into host cell; cell-cell adhesion; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; response to virus; susceptibility to natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity