Testing Data

Mouse anti-Sheep CD8 Monoclonal Antibody | anti-CD8 antibody

MOUSE ANTI SHEEP CD8

Reactivity

Sheep

Applications

Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry

Synonyms

CD8; Monoclonal Antibody; MOUSE ANTI SHEEP CD8; CD8 antibody|38.65; anti-CD8 antibody

Ordering
Cat. Num. AAA225955 For Research Use Only!

Host

Mouse

Reactivity

Sheep

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

38.65

Specificity

CD8

Form/Format

Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Concentration

IgG concentration 1.0mg/ml (varies by lot)

Applicable Applications for anti-CD8 antibody

Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)-Frozen

Application Notes

FC/FACS: 1/50-1/100
FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Species Cross-Reactivity

Bovine, Goat

Immunogen

Ovine efferent lymphocytes.

Preservative Stabilizers

0.09% Sodium Azide (NaN3)

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line

Carrier Free

Yes

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Testing Data

(Staining of sheep peripheral blood lymphocytes with Mouse anti Ovine CD8:FITC .)

Related Product Information for anti-CD8 antibody

Mouse anti Sheep CD8 antibody, clone 38.65 recognizes the ovine CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.
Under reducing conditions, the antigens immunoprecipitated by Mouse anti Sheep CD8 antibody, clone 38.65 migrate at ~33 kDa and ~36 kDa.

References

1. Maddox, J.F. et al. (1985) Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies.Immunology. 55 (4): 739-48.
2. Mackay, C.R. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.Eur J Immunol. 16 (1): 19-25.
3. Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.Cell Immunol. 110 (1): 46-55.
4. Mackay, C.R. et al. (1989) Gamma/delta T cells express a unique surface molecule appearing late during thymic development.Eur J Immunol. 19 (8): 1477-83.
5. Mackay, C.R. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.J Immunol. 136 (5): 1592-9.

Similar Products

Product Notes

The CD8 (Catalog #AAA225955) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI SHEEP CD8 reacts with Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)-Frozen. FC/FACS: 1/50-1/100 FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Loading...

Mouse anti-Sheep CD8 Monoclonal Antibody | anti-CD8 antibody