Testing Data
(Staining of mouse spleen cells probed with Rat anti Mouse CD197: Alexa Fluor 647 conjugated Rat anti Mouse CD197 antibody, clone 4B12)
Rat anti-Mouse CD197 Monoclonal Antibody | anti-CD197 antibody
RAT ANTI MOUSE CD197: RPE
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
CD197; Monoclonal Antibody; RAT ANTI MOUSE CD197: RPE; CD197 antibody|4B12; anti-CD197 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4B12
Specificity
CD197
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE), lyophilized. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Applicable Applications for anti-CD197 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: Max Dilution: Neat
FC/FACS: Use 20ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
FC/FACS: Use 20ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunogen
CCR7 rat basophilic leukaemia (RBL)-2H3 transfectants.
Preservative Stabilizers
0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
1% Bovine Serum Albumin
5% Sucrose
Fusion Partners
Spleen cells from immunised Lou/c rats were fused with cells of the mouse X63.Ag8.653 myeloma cell line
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation and Storage
Store at 4 degree C.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Staining of mouse spleen cells probed with Rat anti Mouse CD197: Alexa Fluor 647 conjugated Rat anti Mouse CD197 antibody, clone 4B12)
Testing Data
(Staining of mouse spleen cells probed with Rat anti Mouse CD197: Alexa Fluor 647 conjugated Rat anti Mouse CD197 antibody, clone 4B12)
Related Product Information for anti-CD197 antibody
Rat anti Mouse CD197 antibody, clone 4B12 recognizes murine C-C chemokine receptor type 7, also known as CD197, Epstein-Barr virus-induced G-protein coupled receptor 1, CCR7 or MIP-3 beta receptor. CD197 is a 354 amino acid ~43 kDa member of the G-protein coupled receptor family. In mice, CD197 is expressed on most naïve T cells, a subset of memory T cells, B cells, mature dendritic cells, NK cells, a subset of thymocytes and CD34+ macrophage progenitor cells.
CD197 is a functional receptor for chemokines CCL21 and CCL19. The receptor plays an important role in regulating the trafficking and homing of lymphocytes and dendritic cells to secondary lymphoid organs.
Rat anti Mouse CD197 antibody, clone 4B12 does not block the binding of the ligand to CD197.
CD197 is a functional receptor for chemokines CCL21 and CCL19. The receptor plays an important role in regulating the trafficking and homing of lymphocytes and dendritic cells to secondary lymphoid organs.
Rat anti Mouse CD197 antibody, clone 4B12 does not block the binding of the ligand to CD197.
References
1. Ritter, U. et al. (2004) Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells.J Leukoc Biol. 76 (2): 472-6.
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Product Notes
The CD197 (Catalog #AAA225959) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RAT ANTI MOUSE CD197: RPE reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD197 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Max Dilution: Neat FC/FACS: Use 20ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD197 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD197, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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