Fluorescein Isothiocyanate (FITC)
(Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD8 (MBS224404) followed by Goat anti Mouse IgG (H/L): FITC (MBS235142))
CD8 Monoclonal Antibody | anti-CD8 antibody
MOUSE ANTI RABBIT CD8
Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Fluorescein Isothiocyanate (FITC)
(Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD8 (MBS224404) followed by Goat anti Mouse IgG (H/L): FITC (MBS235142))
Fluorescein Isothiocyanate (FITC)
(Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD8 (MBS224404) followed by Goat anti Mouse IgG (H/L): FITC (MBS235142))
Fluorescein Isothiocyanate (FITC)
(Published researcher image:Mouse anti Rabbit CD8 antibody, clone 12.C7 (MBS224404) used for the evaluation of CD8 expression on cells from lymph nodes and spleen from rabbits infected with alcelaphine herpesvirus 1 by flow cytometry.Image caption:Analysis of in vivo BrdU incorporation. Rabbits were treated as described in Fig. 1. PBMCs were collected at days 11, 15, 17, 20 and 24 post-inoculation, while mononuclear cells were isolated from popliteal lymph node and spleen at the time of death. Cells were labelled with anti-CD11b, IgM, CD5, CD4 and CD8 mAbs as the primary antibodies. Alexa 633-GAM was used as the secondary antibody.In vivo BrdU incorporation was revealed by immunofluorescent staining as described in Methods. After staining, cells were analysed by flow cytometry.A. Representative flow cytometry dot plots are shown for each double staining, they illustrate the data obtained at day 17 post-infection for the PBMC of rabbits MR17/1 and IR17/1. The data represent the percentages of BrdU positive cells (y-axis) calculated based on the acquisition of 10,000 cells expressing the indicated cell marker (x-axis).B. The percentage of BrdU positive cells amongst the indicated cellular subset was determined and compared between AlHV-1 infected (left column: bold lines; middle and right columns: hatched bars) and mock infected (left column: dotted lines; middle and right columns: open bars) groups (* P)
Fluorescein Isothiocyanate (FITC)
(Published researcher image:Mouse anti Rabbit CD8 antibody, clone 12.C7 (MBS224404) used for the evaluation of CD8 expression on cells from lymph nodes and spleen from rabbits infected with alcelaphine herpesvirus 1 by flow cytometry.Image caption:Analysis of in vivo BrdU incorporation. Rabbits were treated as described in Fig. 1. PBMCs were collected at days 11, 15, 17, 20 and 24 post-inoculation, while mononuclear cells were isolated from popliteal lymph node and spleen at the time of death. Cells were labelled with anti-CD11b, IgM, CD5, CD4 and CD8 mAbs as the primary antibodies. Alexa 633-GAM was used as the secondary antibody.In vivo BrdU incorporation was revealed by immunofluorescent staining as described in Methods. After staining, cells were analysed by flow cytometry.A. Representative flow cytometry dot plots are shown for each double staining, they illustrate the data obtained at day 17 post-infection for the PBMC of rabbits MR17/1 and IR17/1. The data represent the percentages of BrdU positive cells (y-axis) calculated based on the acquisition of 10,000 cells expressing the indicated cell marker (x-axis).B. The percentage of BrdU positive cells amongst the indicated cellular subset was determined and compared between AlHV-1 infected (left column: bold lines; middle and right columns: hatched bars) and mock infected (left column: dotted lines; middle and right columns: open bars) groups (* P)
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Product Notes
The CD8 (Catalog #AAA224404) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD8 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). FC: 1/100 - 1/200 Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the CD8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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