Rat Syndecan-4 ELISA Kit | SDC4 elisa kit

Rat Syndecan-4 ELISA Kit

Cat. Num. AAA764426

• Gene Names: SDC4; SYND4
• Reactivity: Rat
• Synonyms: Syndecan-4; Rat Syndecan-4 ELISA Kit; SDC4/Amphiglycan/Ryudocan/SDC4/amphiglycan/Amphiglycan/MGC22217/ryudocan/Ryudocan core protein/SYND4ryudocan amphiglycan/syndecan 4/syndecan 4 (amphiglycan; ryudocan)/syndecan proteoglycan 4; SDC4 elisa kit

• Reactivity: Rat
• Sequence Length: 198

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SDC4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat SDC4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat SDC4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat SDC4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat SDC4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 38201675
• NCBI GeneID: 6385
• NCBI Accession #: NP_002990.2
• NCBI GenBank Nucleotide #: NM_002999.3
• Molecular Weight: 16,545 Da
• NCBI Official Full Name: syndecan-4
• NCBI Official Synonym Full Names: syndecan 4
• NCBI Official Symbol: SDC4
• NCBI Official Synonym Symbols: SYND4
• NCBI Protein Information: syndecan-4
• UniProt Protein Name: Syndecan-4
• Protein Family: Syndecan
• UniProt Gene Name: SDC4
• UniProt Synonym Gene Names: SYND4
• UniProt Entry Name: SDC4_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

syndecan-4: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Expressed as a homodimer. Promotes cell spreading in a beta(1) integrin-dependent fashion through PKC-alpha and Rho.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: cell surface; costamere; focal adhesion; Golgi lumen; integral to plasma membrane; lipid raft; lysosomal lumen; plasma membrane

Molecular Function: fibronectin binding; protein binding; protein kinase C binding

Biological Process: carbohydrate metabolic process; cell migration; chondroitin sulfate metabolic process; extracellular matrix organization and biogenesis; fat-soluble vitamin metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; inner ear receptor stereocilium organization and biogenesis; neural tube closure; phototransduction, visible light; positive regulation of focal adhesion formation; positive regulation of protein kinase activity; positive regulation of stress fiber formation; retinoid metabolic process; ureteric bud development; vitamin metabolic process; wound healing