Rabbit anti-Human Serine/threonine-protein kinase PAK 2 Polyclonal Antibody | anti-PAK2 antibody

Rabbit anti-human Serine/threonine-protein kinase PAK 2 polyclonal Antibody(PAK2)

Cat. Num. AAA1497290

• Gene Names: PAK2; PAK65; PAKgamma
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: Serine/threonine-protein kinase PAK 2; Polyclonal Antibody; Rabbit anti-human Serine/threonine-protein kinase PAK 2 polyclonal Antibody(PAK2); Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p27; p34; C-t-PAK2; PAK2; anti-PAK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 524

• Applicable Applications for anti-PAK2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Serine/threonine-protein kinase PAK 2 protein (1-212aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1519 / sc-7117 / sc-1872
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Serine/threonine-protein kinase PAK 2 antibody at 2ug/mlLane 1:Jurkat whole cell lysateLane 2:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 58kDaObserved band size: 58kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human endometrial cancer using MBS1497290 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid using MBS1497290 at dilution 1:100)

Related Product Information for anti-PAK2 antibody

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation.

NCBI and Uniprot Product Information

• NCBI GI #: 32483399
• NCBI GeneID: 5062
• NCBI Accession #: NP_002568.2
• NCBI GenBank Nucleotide #: NM_002577.4
• UniProt Accession #: Q13177; Q13154; Q6ISC3
• Molecular Weight: 58,043 Da
• NCBI Official Full Name: serine/threonine-protein kinase PAK 2
• NCBI Official Synonym Full Names: p21 protein (Cdc42/Rac)-activated kinase 2
• NCBI Official Symbol: PAK2
• NCBI Official Synonym Symbols: PAK65; PAKgamma
• NCBI Protein Information: serine/threonine-protein kinase PAK 2
• UniProt Protein Name: Serine/threonine-protein kinase PAK 2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK2
• UniProt Synonym Gene Names: PAK-2; p27; p34
• UniProt Entry Name: PAK2_HUMAN

NCBI Description

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008]

Uniprot Description

PAK2: a protein kinase of the STE20 family. Critical effector linking RhoGTPases to cytoskeleton reorganization and nuclear signaling. Activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE20 family; PAKA subfamily

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: cytoplasm; cytosol; nucleoplasm; perinuclear region of cytoplasm; plasma membrane

Molecular Function: ATP binding; identical protein binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; protein tyrosine kinase activator activity; Rac GTPase binding

Biological Process: actin cytoskeleton organization and biogenesis; apoptosis; axon guidance; cell migration; cell structure disassembly during apoptosis; innate immune response; negative regulation of apoptosis; negative regulation of protein kinase activity; peptidyl-serine phosphorylation; phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; programmed cell death; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of defense response to virus by virus; regulation of growth; regulation of MAPKKK cascade; regulation of mitotic cell cycle; Rho protein signal transduction; signal transduction; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; stress-activated protein kinase signaling pathway; T cell costimulation; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction