Mouse anti-Human CD53 Monoclonal Antibody | anti-CD53 antibody

Anti-Hu CD53 Pacific Blue

• Gene Names: CD53; MOX44; TSPAN25
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD53; Monoclonal Antibody; Anti-Hu CD53 Pacific Blue; Tetraspanin-25; MOX44; TSPAN25; CD53 molecule; anti-CD53 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: MEM-53
• Specificity: The antibody MEM-53 reacts with an extracellular epitope of CD53, a 32-40kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15kDa using endoglycosidase F).
• Purity/Purification: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Pacific Blue. Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD53 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Immunogen: Leukocytes of pacient suffering from a LGL-type leukemia.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (intracellular staining) of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) Pacific BlueTM.)

Related Product Information for anti-CD53 antibody

CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.

References

*Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11, URL: http://www.ncbi.nlm.nih.gov/pubmed/26396269,*Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1;85(9):2402-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/7537112,*Szollosi J, Horejsi V, Bene L, Angelisova P, Damjanovich S: Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol. 1996 Oct 1;157(7):2939-46., URL: https://www.ncbi.nlm.nih.gov/pubmed/8816400dopt=A,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL:,*Rasmussen AM, Blomhoff HK, Stokke T, Horejsi V, Smeland EB: Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol. 1994 Dec 1;153(11):4997-5007., URL: https://www.ncbi.nlm.nih.gov/pubmed/7963560dopt=A,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL:,*Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha). 1989;35(5):289-97., URL: https://www.ncbi.nlm.nih.gov/pubmed/2534480dopt=A
*Yunta M, Rodríguez-Barbero A, Arévalo MA, López-Novoa JM, Lazo PA: Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primary cultures of mesangial cells. Kidney Int. 2003 Feb;63(2):534-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/12631118ordin,*Yunta M, Lazo PA: Apoptosis protection and survival signal by the CD53 tetraspanin antigen. Oncogene. 2003 Feb 27;22(8):1219-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/12606948ordin,*Kim TR, Yoon JH, Kim YC, Yook YH, Kim IG, Kim YS, Lee H, Paik SG: LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress. Mol Cells. 2004 Feb 29;17(1):125-31., URL: https://www.ncbi.nlm.nih.gov/pubmed/15055538ordin,*Puls KL, Hogquist KA, Reilly N, Wright MD: CD53, a thymocyte selection marker whose induction requires a lower affinity TCR-MHC interaction than CD69, but is up-regulated with slower kinetics. Int Immunol. 2002 Mar;14(3):249-58., URL: https://www.ncbi.nlm.nih.gov/pubmed/11867561ordin,*Mollinedo F, Martín-Martín B, Gajate C, Lazo PA: Physiological activation of human neutrophils down-regulates CD53 cell surface antigen. J Leukoc Biol. 1998 Jun;63(6):699-706., URL: https://www.ncbi.nlm.nih.gov/pubmed/9620662ordina,*Angelisova P, Vlcek C, Stefanova I, Lipoldova M, Horejsi V: The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics. 1990;32(4):281-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/1700763dopt=A,*Olweus J, Lund-Johansen F, Horejsi V: CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol. 1993 Jul 15;151(2):707-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/8335905dopt=A

NCBI and Uniprot Product Information

• NCBI GI #: 10834972
• NCBI GeneID: 963
• NCBI Accession #: NP_000551.1
• NCBI GenBank Nucleotide #: NM_000560.3
• UniProt Accession #: P19397; Q5U0D6; B2R905
• Molecular Weight: 24,341 Da
• NCBI Official Full Name: leukocyte surface antigen CD53
• NCBI Official Synonym Full Names: CD53 molecule
• NCBI Official Symbol: CD53
• NCBI Official Synonym Symbols: MOX44; TSPAN25
• NCBI Protein Information: leukocyte surface antigen CD53; CD53 antigen; CD53 glycoprotein; CD53 tetraspan antigen; antigen MOX44 identified by monoclonal antibody MRC-OX44; cell surface antigen; cell surface glycoprotein CD53; tetraspanin-25; transmembrane glycoprotein; tspan-25
• UniProt Protein Name: Leukocyte surface antigen CD53
• Protein Family: Leukocyte surface antigen
• UniProt Gene Name: CD53
• UniProt Synonym Gene Names: MOX44; TSPAN25; Tspan-25
• UniProt Entry Name: CD53_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

CD53: May be involved in growth regulation in hematopoietic cells. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: cell surface; plasma membrane; integral to membrane; immunological synapse; intercellular junction

Molecular Function: protein binding

Biological Process: signal transduction