Immunohistochemistry (IHC)
(MBS9609336 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit MAGE1 Polyclonal Antibody | anti-MAGE1 antibody
MAGE1 Antibody
Gene Names
MAGEE1; HCA1; DAMAGE
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MAGE1; Polyclonal Antibody; MAGE1 Antibody; AI847422; Alpha-dystrobrevin-associated MAGE Protein; DAMAGE; HCA1; Hepatocellular carcinoma-associated protein 1; MAGE-E1 antigen; MAGE1_HUMAN; MAGEE1; Melanoma-associated antigen E1; mMage-e1; RGD1560259; RP23-82A12.1; RP4-596C3.1; anti-MAGE1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MAGE1 antibody detects endogenous levels of total MAGE1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
957
Applicable Applications for anti-MAGE1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human MAGE1
Subcellular Location
Cytoplasm > Perinuclear Region. Nucleus. Cell Membrane. In the skeletal muscle, found at the postsynaptic membrane and is associated with a subset of myonuclei. May reside within nuclei and/or in perinuclear compartments. In peripheral nerves, co-localizes with DTNA in the Schwann Cell Membrane.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9609336 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609336 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of MAGE1 using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis of MAGE1 using K562 whole cell lysates)
Related Product Information for anti-MAGE1 antibody
Function: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.
Subunit Structure: Interacts with DTNA (By similarity). Interacts with TRIM28.
Subunit Structure: Interacts with DTNA (By similarity). Interacts with TRIM28.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 103 kDa
Predicted: 104 kDa
Predicted: 104 kDa
NCBI Official Full Name
melanoma-associated antigen E1
NCBI Official Synonym Full Names
MAGE family member E1
NCBI Official Symbol
MAGEE1
NCBI Official Synonym Symbols
HCA1; DAMAGE
NCBI Protein Information
melanoma-associated antigen E1
UniProt Protein Name
Melanoma-associated antigen E1
UniProt Gene Name
MAGEE1
UniProt Synonym Gene Names
HCA1; KIAA1587; DAMAGE
NCBI Description
This gene encodes an alpha-dystrobrevin-associated MAGE (melanoma-associated antigen) protein, which is a member of the MAGE family. The protein contains a nuclear localization signal in the N-terminus, 30 12-amino acid repeats beginning at nt 60 with the consensus sequence ASEGPSTSVLPT, and two MAGE domains in the C-terminus. It may play a signaling role in brain, muscle, and peripheral nerve. This gene is located on X chromosome in a region containing loci linked to cognitive disability. [provided by RefSeq, Mar 2010]
Uniprot Description
May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.
Research Articles on MAGE1
1. Data suggest that lipid homeostasis may be altered in carriers of selected HCA1 missense variants.
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Product Notes
The MAGE1 magee1 (Catalog #AAA9609336) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAGE1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAGE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the MAGE1 magee1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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