Flow Cytometry (FC/FACS)
(Separation of MCF-7 cells stained using anti-CD51/CD61 (23c3) purified antibody (concentration in sample 1,7 ug/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).)
Mouse CD51/CD61 Monoclonal Antibody | anti-CD51/CD61 antibody
Anti-Hu CD51/CD61 Purified
Reactivity
Human, Chicken
Applications
Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified by protein-A affinity chromatography
Synonyms
CD51/CD61; Monoclonal Antibody; Anti-Hu CD51/CD61 Purified; VNRA; MSK8; ITGB3; ITGAV; Integrin alpha5beta3; OFA; VR; anti-CD51/CD61 antibody
Host
Mouse
Reactivity
Human, Chicken
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
23C6
Specificity
The mouse monoclonal antibody 23C6 recognizes an extracellular epitope on CD51/CD61 complex, that is expressed mainly on human osteoclasts, but also e.g. on placenta, or melanoma cell lines. The epitope is native and sensitive to fixation. In chicken this antibody can be used to selectively identify the thrombocytes.
Purity/Purification
Purified by protein-A affinity chromatography
Form/Format
Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD51/CD61 antibody
Immunoprecipitation (IP), Immunohistochemistry-Frozen (IHC-F), Flow Cytometry (FC/FACS) (QC tested)
Application Notes
FC/FACS: Recommended dilution: 2-6ug/ml
Negative Species
Rabbit, Pig, Cow
Immunogen
A cell suspension containing osteoclasts from osteoclastomas
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.
Flow Cytometry (FC/FACS)
(Separation of MCF-7 cells stained using anti-CD51/CD61 (23c3) purified antibody (concentration in sample 1,7 ug/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).)
Flow Cytometry (FC/FACS)
(Separation of MCF-7 cells stained using anti-CD51/CD61 (23c3) purified antibody (concentration in sample 1,7 ug/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).)
Related Product Information for anti-CD51/CD61 antibody
CD51/CD61 (integrin alpha5beta3), also known as osteoclast Functional Application (FUNC)tional antigen, serves as a vitronectin receptor, and binds also to fibronectin, fibrinogen, thrombospondin, osteopontin, collagen, and von Willebrand factor. Expression of this antigen increases with melanoma progression. In healthy individuals CD51/CD61 is expressed mainly on osteoclasts, placenta, and endothelial cells, at lower levels on platelets and macrophages.
References
*Viertlboeck BC, Göbel TW: Chicken thrombocytes express the CD51/CD61 integrin. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):137-41, URL: https://www.ncbi.nlm.nih.gov/pubmed/term=chicken+thrombocytes+express+cd51,*Yamamoto T, Kurihara N, Yamaoka K, Ozono K, Okada M, Yamamoto K, Matsumoto S, Michigami T, Ono J, Okada S: Bone marrow-derived osteoclast-like cells from a patient with craniometaphyseal dysplasia lack expression of osteoclast-reactive vacuolar proton pump. J Clin Invest. 1993 Jan;91(1):362-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=yamamoto+t+kurihara+n+yamaoka+k,*Leukocyte Typing IV., Knapp WB. et al. (Eds.), Oxford University Press (1989)., URL:,*Davies J, Warwick J, Totty N, Philp R, Helfrich M, Horton M: The osteoclast Functional Application (FUNC)tional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor. J Cell Biol. 1989 Oct;109(4 Pt 1):1817-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=davies+j+1989+1817,*Barua A, Bitterman P, Bahr JM, Bradaric MJ, Hales DB, Luborsky JL, Abramowicz JS: Detection of tumor-associated neoangiogenesis by Doppler ultrasonography during early-stage ovarian cancer in laying hens: a preclinical model of human spontaneous ovarian cancer. J Ultrasound Med. 2010 Feb;29(2):173-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=barua+2010+173
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Product Notes
The CD51/CD61 (Catalog #AAA1801806) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD51/CD61 Purified reacts with Human, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's CD51/CD61 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunohistochemistry-Frozen (IHC-F), Flow Cytometry (FC/FACS) (QC tested). FC/FACS: Recommended dilution: 2-6ug/ml. Researchers should empirically determine the suitability of the CD51/CD61 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD51/CD61, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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