Mouse CD61r Monoclonal Antibody | anti-CD61r antibody

Anti-Hu CD61 PerCP-Cy5.5

Cat. Num. AAA1802237

• Gene Names: ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16
• Reactivity: Human, Non-human primates
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with tandem dye PerCP-Cy5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD61r; Monoclonal Antibody; Anti-Hu CD61 PerCP-Cy5.5; integrin beta 3; ITGB3; GP3A; integrin subunit beta 3; anti-CD61r antibody

• Host: Mouse
• Reactivity: Human, Non-human primates
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: VIPL2
• Specificity: The mouse monoclonal antibody VIPL2 recognizes an extracellular epitope of CD61, a 90-110kDa transmembrane glycoprotein of integrin family, expressed on platelets, megacaryocytes, osteoclasts, endothelial cells and other cell types, including leucocytes and smooth muscle cells.
• Purity/Purification: The purified antibody is conjugated with tandem dye PerCP-Cy5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD61r antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: PerCP-Cy5.5
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD61 (VIPL2) PerCP-Cy™5.5 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human thrombocytes stained using anti-human CD61 (VIPL2) PerCP-Cy™5.5 antibody (4 ul reagent / 100 ul of peripheral whole blood) from unstained trombocytes (black-dashed) in flow cytometry analysis.)

Related Product Information for anti-CD61r antibody

CD61 (beta3 integrin) is a transmembrane glycoprotein, which associates with CD41 or CD51 molecules to form heterodimeric adhesion receptores. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. It binds to fibronectin, fibrinogen and von Willebrand factor, and is involved in platelet aggregation. CD51/CD61 complex has similar binding properties and is involved in modulating migration and survival of angiogenic endothelial cells.

References

*Roberts MS, Woods AJ, Dale TC, Van Der Sluijs P, Norman JC: Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of alpha v beta 3 and alpha 5 beta 1 integrins. Mol Cell Biol. 2004 Feb;24(4):1505-15., URL: http://www.ncbi.nlm.nih.gov/pubmed/14749368,*Barrett L, Dai C, Gamberg J, Gallant M, Grant M: Circulating CD14-CD36+ peripheral blood mononuclear cells constitutively produce interleukin-10. J Leukoc Biol. 2007 Jul;82(1):152-60., URL: http://www.ncbi.nlm.nih.gov/pubmed/17412916,*Ciarlet M, Crawford SE, Cheng E, Blutt SE, Rice DA, Bergelson JM, Estes MK: VLA-2 (alpha2beta1) integrin promotes rotavirus entry into cells but is not necessary for rotavirus attachment. J Virol. 2002 Feb;76(3):1109-23., URL: http://www.ncbi.nlm.nih.gov/pubmed/11773387,*Williams CA, Mondal D, Agrawal KC: The HIV-1 Tat protein enhances megakaryocytic commitment of K562 cells by facilitating CREB transcription factor coactivation by CBP. Exp Biol Med (Maywood). 2005 Dec;230(11):872-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/16339753,*Mondal D, Williams CA, Ali M, Eilers M, Agrawal KC: The HIV-1 Tat protein selectively enhances CXCR4 and inhibits CCR5 expression in megakaryocytic K562 cells. Exp Biol Med (Maywood). 2005 Oct;230(9):631-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/16179731,*Dzionek A, Fuchs A, Schmidt P, Cremer S, Zysk M, Miltenyi S, Buck DW, Schmitz J: BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol. 2000 Dec 1;165(11):6037-46., URL: https://www.ncbi.nlm.nih.gov/pubmed/11086035

NCBI and Uniprot Product Information

• NCBI GI #: 47078292
• NCBI GeneID: 3690
• NCBI Accession #: NP_000203.2
• NCBI GenBank Nucleotide #: NM_000212.2
• UniProt Accession #: P05106; O15495; Q12806; Q13413; Q14648; Q16499; A0PJW2; D3DXJ8
• Molecular Weight: 87,058 Da
• NCBI Official Full Name: integrin beta-3
• NCBI Official Synonym Full Names: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• NCBI Official Symbol: ITGB3
• NCBI Official Synonym Symbols: GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16
• NCBI Protein Information: integrin beta-3; platelet membrane glycoprotein IIIa
• UniProt Protein Name: Integrin beta-3
• UniProt Gene Name: ITGB3
• UniProt Synonym Gene Names: GP3A; GPIIIa
• UniProt Entry Name: ITB3_HUMAN

NCBI Description

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: filopodium membrane; platelet alpha granule membrane; focal adhesion; cell surface; microvillus membrane; integral to plasma membrane; plasma membrane; melanosome; integrin complex; nucleus; receptor complex

Molecular Function: identical protein binding; protein binding; protease binding; extracellular matrix binding; vascular endothelial growth factor receptor 2 binding; fibronectin binding; platelet-derived growth factor receptor binding; protein disulfide isomerase activity; cell adhesion molecule binding; receptor activity

Biological Process: negative regulation of lipid transport; axon guidance; entry of virus into host cell; extracellular matrix organization and biogenesis; protein folding; wound healing; cell-matrix adhesion; smooth muscle cell migration; mesodermal cell differentiation; negative chemotaxis; positive regulation of vascular endothelial growth factor receptor signaling pathway; platelet degranulation; cell-substrate junction assembly; cell growth; cell adhesion; integrin-mediated signaling pathway; platelet activation; regulation of bone resorption; cell migration; negative regulation of lipoprotein metabolic process; angiogenesis involved in wound healing; cell-substrate adhesion; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of low-density lipoprotein receptor biosynthetic process; activation of protein kinase activity; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; tube development; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Myocardial Infarction, Susceptibility To; Glanzmann Thrombasthenia; Bleeding Disorder, Platelet-type, 16