Rat CD51 Monoclonal Antibody | anti-CD51 antibody

RAT ANTI MOUSE CD51

Cat. Num. AAA214310

• Gene Names: Itgav; CD51; 1110004F14Rik; 2610028E01Rik; D430040G12Rik
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CD51; Monoclonal Antibody; RAT ANTI MOUSE CD51; anti-CD51 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: RMV-7
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 1044

• Applicable Applications for anti-CD51 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.; Immunohistology - Frozen: Minimum Dilution: 1/25; Maximum Dilution: 1/100; Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/100
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: Cultured LAK cells from Balb/c mice.; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CD51 antibody

Rat anti Mouse CD51 antibody, clone RMV-7 recognizes murine CD51, a 140 kD alpha subunit of the vitronectin receptor, which is otherwise known as the integrin alpha v chain. CD51 can form heterodimers at the cell surface with a variety of beta integrins including CD29 and CD61. Heterodimers of CD51/CD61 function as a receptor for vitronectin, and a wide array of RGD-containing proteins including fibronectin, fibrinogen, von Willebrand factor, laminin, thrombospondin and osteopontin. CD51/CD61 is primarily expressed on myeloid cells and activated T-cells. Alpha-V integrins may play a role in embryo implantation, angiogenesis and wound healing. The RMV-7 antibody has been reported to block binding of CD51 to vitronectin, fibronectin, and CD31 in some cell types, as well as blocking LAK cell cytotoxicity (Takahashi et al. 1990).

NCBI and Uniprot Product Information

• NCBI GI #: 154240716
• NCBI GeneID: 16410
• NCBI Accession #: NP_032428.2
• NCBI GenBank Nucleotide #: NM_008402.3
• UniProt Accession #: P43406; A2AKI6
• Molecular Weight: 115,360 Da
• NCBI Official Full Name: integrin alpha-V
• NCBI Official Synonym Full Names: integrin alpha V
• NCBI Official Symbol: Itgav
• NCBI Official Synonym Symbols: CD51; 1110004F14Rik; 2610028E01Rik; D430040G12Rik
• NCBI Protein Information: integrin alpha-V; vitronectin receptor alpha polypeptide (VNRA); vitronectin receptor subunit alpha
• UniProt Protein Name: Integrin alpha-V
• UniProt Gene Name: Itgav
• UniProt Entry Name: ITAV_MOUSE

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 1, beta 3, beta 6 and beta 8. The heterodimer of alpha v and beta 3 forms the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. In mouse, deficiency of this gene is associated with defects in vascular morphogenesis in the brain and early post-natal death. [provided by RefSeq, May 2013]

Uniprot Description

ITGAV: The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Belongs to the integrin alpha chain family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: filopodium membrane; cell surface; focal adhesion; membrane; microvillus membrane; integral to membrane; plasma membrane; intracellular; cytosol; nucleus; integrin complex; external side of plasma membrane

Molecular Function: voltage-gated calcium channel activity; opsonin binding; insulin-like growth factor I binding; protease binding; protein kinase C binding; extracellular matrix binding; protein heterodimerization activity; metal ion binding; fibronectin binding; peptide binding; receptor binding

Biological Process: negative regulation of lipid transport; focal adhesion formation; entry of virus into host cell; positive regulation of cell adhesion; T cell activation; cell-matrix adhesion; multicellular organismal development; negative chemotaxis; gonad morphogenesis; regulation of phagocytosis; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; angiogenesis; cell growth; cell adhesion; inflammatory response; cell differentiation; integrin-mediated signaling pathway; blood vessel development; cell migration; regulation of bone resorption; negative regulation of lipoprotein metabolic process; detection of molecule of bacterial origin; cell-substrate adhesion; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; fertilization; negative regulation of low-density lipoprotein receptor biosynthetic process; release of sequestered calcium ion into cytosol; positive regulation of osteoblast proliferation; endothelial cell migration; apoptotic cell clearance; positive regulation of cell migration