Rabbit anti-Human, Mouse YIPF5 Polyclonal Antibody | anti-YIPF5 antibody

YIPF5, NT (YIPF5, FINGER5, YIP1A, Protein YIPF5, Five-pass transmembrane protein localizing in the Golgi apparatus and the endoplasmic reticulum 5, Smooth muscle cell-associated protein 5, YIP1 family member 5, YPT-interacting protein 1 A) (FITC)

Cat. Num. AAA6364306

• Gene Names: YIPF5; SB140; SMAP5; YIP1A; SMAP-5; FinGER5
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: YIPF5; Polyclonal Antibody; NT (YIPF5; FINGER5; YIP1A; Protein YIPF5; Five-pass transmembrane protein localizing in the Golgi apparatus and the endoplasmic reticulum 5; Smooth muscle cell-associated protein 5; YIP1 family member 5; YPT-interacting protein 1 A) (FITC); YPT-interacting protein 1 A; anti-YIPF5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-YIPF5 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: YIPF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28~57 amino acids from the N-terminal region of human YIPF5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of YIPF5 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). YIPF5 (arrow) was detected using the purified Pab;)

Western Blot (WB)

(Western blot analysis of YIPF5 Antibody (N-term) in CHO cell line lysates (35ug/lane). YIPF5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(YIPF5 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YIPF5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-YIPF5 antibody

Plays a role in transport between endoplasmic reticulum and Golgi.

Product Categories/Family for anti-YIPF5 antibody

Antibodies; Transmembrane Proteins

References

Koskinen, L.L., et al. Tissue Antigens 74(5):408-416(2009). Kano, F., et al. J. Cell. Sci. 122 (PT 13), 2218-2227 (2009). Yoshida, Y., et al. Exp. Cell Res. 314(19):3427-3443(2008). Jin, C., et al. Biochem. Biophys. Res. Commun. 334(1):16-22(2005). Stolle, K., et al. Gene 351, 119-130 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 68226422
• NCBI GeneID: 81555
• NCBI Accession #: NP_001020118.1
• NCBI GenBank Nucleotide #: NM_001024947.3
• UniProt Accession #: Q969M3; Q4VSN6; Q53EX4; Q8NHE5; Q9H338; Q9H3U4; D3DQF5
• Molecular Weight: 29,107 Da
• NCBI Official Full Name: protein YIPF5 isoform a
• NCBI Official Synonym Full Names: Yip1 domain family member 5
• NCBI Official Symbol: YIPF5
• NCBI Official Synonym Symbols: SB140; SMAP5; YIP1A; SMAP-5; FinGER5
• NCBI Protein Information: protein YIPF5
• UniProt Protein Name: Protein YIPF5
• Protein Family: Protein
• UniProt Gene Name: YIPF5
• UniProt Synonym Gene Names: FINGER5; YIP1A; SMAP-5

Uniprot Description

SMAP-5: Plays a role in transport between endoplasmic reticulum and Golgi. Belongs to the YIP1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: COPII-coated ER to Golgi transport vesicle; endoplasmic reticulum; endoplasmic reticulum exit site; endoplasmic reticulum membrane; Golgi apparatus; integral component of membrane; intracellular membrane-bound organelle; nucleoplasm

Molecular Function: protein binding

Biological Process: protein transport; vesicle-mediated transport