Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD45 expression on human whole blood lymphocytes. Cells were stained with PerCP-conjugated anti-Human CD45. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Mouse anti-Human CD45 Monoclonal Antibody | anti-PTPRC antibody
Anti-CD45 Antibody (PerCP), Mouse Monoclonal
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD45; Monoclonal Antibody; Anti-CD45 Antibody (PerCP); Mouse Monoclonal; CD45/PTPRC Antibody (PerCP); Mouse MAb; protein tyrosine phosphatase; receptor type; C; Anti-B220 Antibody; Anti-CD45 Antibody; Anti-CD45R Antibody; Anti-GP180 Antibody; Anti-L-CA Antibody; Anti-LCA Antibody; Anti-LY5 Antibody; Anti-T200 Antibody; anti-PTPRC antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
05
Specificity
Human CD45/PTPRC
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
5ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-PTPRC antibody
Flow Cytometry (FC/FACS/FCM)
Immunogen
Recombinant Human CD45/PTPRC Protein
Conjugation
PerCP
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD45/PTPRC (rh CD45/PTPRC) and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD45 expression on human whole blood lymphocytes. Cells were stained with PerCP-conjugated anti-Human CD45. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD45 expression on human whole blood lymphocytes. Cells were stained with PerCP-conjugated anti-Human CD45. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-PTPRC antibody
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.
Product Categories/Family for anti-PTPRC antibody
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Irie-Sasaki J, et al. (2001) CD45 is a JAK phosphatase and negatively regulates cytokine receptor signaling. Nature. 409: 349-54.
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The PTPRC (Catalog #AAA8100767) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD45 Antibody (PerCP), Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD45 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the PTPRC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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