Rabbit CD45 Polyclonal Antibody | anti-PTPRC antibody

Anti-CD45 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101644

• Gene Names: Ptprc; Lca; RT7; CD45; L-CA; T200
• Reactivity: Human; Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: CD45; Polyclonal Antibody; Anti-CD45 Antibody; Rabbit Polyclonal; CD45/PTPRC Antibody; Rabbit PAb; Antigen Affinity Purified; protein tyrosine phosphatase; receptor type; C; Anti-B220 Antibody; Anti-CD45R Antibody; Anti-GP180 Antibody; Anti-L-CA Antibody; Anti-LCA Antibody; Anti-LY5 Antibody; Anti-T200 Antibody; anti-PTPRC antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CD45
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PTPRC antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:250-1:1000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human CD45/PTPRC
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CD45/PTPRC, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse Ptprc in mouse brain with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse Ptprc in mouse spleen with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-PTPRC antibody

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.

Product Categories/Family for anti-PTPRC antibody

Phosphatases and Regulators; B Cell Receptor Signaling Pathway

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Irie-Sasaki J, et al. (2001) CD45 is a JAK phosphatase and negatively regulates cytokine receptor signaling. Nature. 409: 349-54.

NCBI and Uniprot Product Information

• NCBI GI #: 158138490
• NCBI GeneID: 24699
• NCBI Accession #: NP_001103357.1
• NCBI GenBank Nucleotide #: NM_001109887.1
• UniProt Accession #: P04157
• Molecular Weight: 134,182 Da
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase C isoform 3
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, C
• NCBI Official Symbol: Ptprc
• NCBI Official Synonym Symbols: Lca; RT7; CD45; L-CA; T200
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C; Protein tyrosine phosphatase, receptor-type, c polypeptide; T200 glycoprotein; leucocyte common antigen; leukocyte common antigen A; leukocyte common antigen B
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: Ptprc
• UniProt Synonym Gene Names: L-CA
• UniProt Entry Name: PTPRC_RAT

NCBI Description

member of a family of heavily glycosylated leukocyte cell surface glycoproteins; displays extensive O-glycosylation [RGD, Feb 2006]

Uniprot Description

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine; EC 3.1.3.48

Cellular Component: internal side of plasma membrane; cell surface; focal adhesion; membrane; integral to plasma membrane; integral to membrane; plasma membrane; intracellular; cytosol; lipid raft; external side of plasma membrane

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase regulator activity; protein kinase binding

Biological Process: B cell proliferation; positive regulation of isotype switching to IgG isotypes; activation of MAPK activity; regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of T cell mediated cytotoxicity; regulation of cell cycle; protein amino acid dephosphorylation; T cell receptor signaling pathway; T cell proliferation; leukocyte adhesion; B cell receptor signaling pathway; positive regulation of MAPKKK cascade; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; response to gamma radiation; positive regulation of T cell mediated immunity; positive regulation of T cell proliferation; negative regulation of protein amino acid autophosphorylation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of gamma-delta T cell differentiation; defense response to virus; positive thymic T cell selection; T cell differentiation; regulation of B cell receptor signaling pathway; positive regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of peptidyl-tyrosine phosphorylation; substrate-bound cell migration, cell release from substrate; negative thymic T cell selection; positive regulation of antigen receptor-mediated signaling pathway; bone marrow development; heterotypic cell-cell adhesion; stem cell development; positive regulation of T cell differentiation; dephosphorylation; B cell differentiation; release of sequestered calcium ion into cytosol; regulation of B cell differentiation; negative regulation of protein kinase activity; positive regulation of alpha-beta T cell proliferation; hemopoietic progenitor cell differentiation; immunoglobulin biosynthetic process