Rabbit anti-Human CD37 Monoclonal Antibody | anti-CD37 antibody

Anti-CD37 [G28-1]

Cat. Num. AAA488871

• Gene Names: CD37; GP52-40; TSPAN26
• Reactivity: Human
• Applications: Depletion
• Purity: Purified antibody. Protein A affinity purified
• Synonyms: CD37; Monoclonal Antibody; Anti-CD37 [G28-1]; TSPAN-26; Leukocyte antigen CD37; Tetraspanin-26; tetraspanin; Tspan-26; anti-CD37 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG, kappa
• Clone Number: G28-1
• Specificity: This antibody recognizes CD37, which is a cell surface protein, present mostly on mature B cells and B cell malignancies.
• Purity/Purification: Purified antibody. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD37 antibody: Depletion
• Application Notes: G28-1 antibody was used to deplete normal and malignant B cells (Hayden-Ledbetter, unpublishesd). This antibody and antibody fragments derived therefrom have been used in the research and trials on the generation of novel immunotherapuetic agents, alternative to anti-CD20 therpaies (rituximab) (Robak and Robak, 2014).
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of fixed Daudi cells with anti-CD37 antibody G28-1 (MBS488871) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of G28-1 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometry using the Anti-CD37 antibody G28-1 (MBS488871). Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of G28-1 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

Related Product Information for anti-CD37 antibody

Recombinant monoclonal antibody to CD37. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma G28-1.
This antibody was raised by immunizing mice with CD37.

References

Ledbetter et al. Monoclonal antibodies to a new gp40-45 (CD37) B cell associated cluster group modulate B cell proliferation. LEUCOCYTE TYPING III: WHITE CELL DIFFERENTIATION ANTIGENS: 339-341; Oxford University Press, USA (1987)
Note on publication: This article decribes the generation of the G28-1 antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 91807110
• NCBI GeneID: 951
• NCBI Accession #: NP_001035120.1
• NCBI GenBank Nucleotide #: NM_001040031.1
• UniProt Accession #: P11049; Q3KPF9; B4DVC1
• Molecular Weight: 22,563 Da
• NCBI Official Full Name: leukocyte antigen CD37 isoform B
• NCBI Official Synonym Full Names: CD37 molecule
• NCBI Official Symbol: CD37
• NCBI Official Synonym Symbols: GP52-40; TSPAN26
• NCBI Protein Information: leukocyte antigen CD37
• UniProt Protein Name: Leukocyte antigen CD37
• Protein Family: Leukocyte antigen
• UniProt Gene Name: CD37
• UniProt Synonym Gene Names: TSPAN26; Tspan-26
• UniProt Entry Name: CD37_HUMAN

Uniprot Description

CD37: is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It may play a role in T-cell-B-cell interactions. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: immunological synapse; integral to plasma membrane; membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction