Rabbit ETFA Polyclonal Antibody | anti-ETFA antibody

ETFA Polyclonal Antibody

Cat. Num. AAA9128430

• Gene Names: ETFA; EMA; GA2; MADD
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: ETFA; Polyclonal Antibody; ETFA Polyclonal Antibody; anti-ETFA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 284

• Applicable Applications for anti-ETFA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluoresence (IF)
• Application Notes: WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:100
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human ETFA
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ETFA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate using ETFA antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using ETFA antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using ETFA antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-ETFA antibody

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 4503607
• NCBI GeneID: 2108
• NCBI Accession #: NP_000117.1
• NCBI GenBank Nucleotide #: NM_000126.3
• UniProt Accession #: P13804; Q53XN3; B4DT43
• Molecular Weight: 35kDa
• NCBI Official Full Name: electron transfer flavoprotein subunit alpha, mitochondrial isoform a
• NCBI Official Synonym Full Names: electron-transfer-flavoprotein, alpha polypeptide
• NCBI Official Symbol: ETFA
• NCBI Official Synonym Symbols: EMA; GA2; MADD
• NCBI Protein Information: electron transfer flavoprotein subunit alpha, mitochondrial
• UniProt Protein Name: Electron transfer flavoprotein subunit alpha, mitochondrial
• Protein Family: Electron transfer flavoprotein
• UniProt Gene Name: ETFA
• UniProt Synonym Gene Names: Alpha-ETF
• UniProt Entry Name: ETFA_HUMAN

NCBI Description

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ETFA: The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Defects in ETFA are the cause of glutaric aciduria type 2A (GA2A); also known as glutaricaciduria IIA. GA2A is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Belongs to the ETF alpha-subunit/FixB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 15q23-q25

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: electron carrier activity; FAD binding; oxidoreductase activity

Biological Process: cellular metabolic process

Disease: Multiple Acyl-coa Dehydrogenase Deficiency