Mouse anti-Human CD37 Monoclonal Antibody | anti-CD37 antibody
Mouse anti-Human CD37, FITC Conjugated mAb
Gene Names
CD37; GP52-40; TSPAN26
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA
Synonyms
CD37; Monoclonal Antibody; Mouse anti-Human CD37; FITC Conjugated mAb; anti-CD37 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
4AIPO-24
Form/Format
Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Sequence Length
281
Applicable Applications for anti-CD37 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA (EIA)
Experimental Methods
1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]
2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
Related Product Information for anti-CD37 antibody
4AIPO-24 reacts with CD37 (a.k.a. gp52-40), a 40-52 kDa molecule, which is strongly expressed on B cells from the pre-B cell stage, but not on plasma cells. It is also present at low levels on some T cells, monocytes and granulocytes. CD37 is a stable marker for malignancies derived from mature B cells, such as B-CLL, HCL and all types of B-NHL. CD37 is involved in signal transduction.* CD37 is stable marker for malignancies derived from mature B cells: B-CLL. HCL and B-NHL.
References
1.Kishimoto, T. et al. (1998) Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London. 2.Barclay, A. N. et al. (1993) The Leucocyte Antigen FactsBook, Academic Press Inc., San Diego, California. 3.Wright, MD and Tomlinson, MD (1994) Immunogenetics 15: 588.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,563 Da
NCBI Official Full Name
CD37, partial
NCBI Official Synonym Full Names
CD37 molecule
NCBI Official Symbol
CD37
NCBI Official Synonym Symbols
GP52-40; TSPAN26
NCBI Protein Information
leukocyte antigen CD37
UniProt Protein Name
Leukocyte antigen CD37
Protein Family
UniProt Gene Name
CD37
UniProt Synonym Gene Names
TSPAN26; Tspan-26
NCBI Description
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It may play a role in T-cell-B-cell interactions. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Research Articles on CD37
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Product Notes
The CD37 cd37 (Catalog #AAA8504241) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Human CD37, FITC Conjugated mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD37 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA (EIA). Researchers should empirically determine the suitability of the CD37 cd37 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD37, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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