Mouse anti-Human CD37 Monoclonal Antibody | anti-CD37 antibody

anti-human CD37-Biotin

Cat. Num. AAA666554

• Gene Names: CD37; GP52-40; TSPAN26
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD37; Monoclonal Antibody; anti-human CD37-Biotin; Monoclonal anti-human CD37/Biotin; anti-CD37 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: IPO-24
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 281

• Applicable Applications for anti-CD37 antibody: Flow Cytometry (FC/FACS)
• Performance: Five x 105 cultured human Raji cells per tube were incubated 45 minutes on ice with 80 ul of anti-CD37/Biotin at 5 ug/ml. Cells were then washed twice and incubated 45 minutes with 2 degree reagent Streptavidin/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.9 log10 fluorescent units when compared to a Mouse IgG2b/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD37 antibody.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Human spleen cells from a patient with hairy cell leukemia
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD37 antibody

Information: Human CD37 is a member of the tetraspan family of cell surface molecules that apparantly are involved in signal transduction or transporter system complexes. CD37 is strongly expressed on B lymphocytes (sIg-positive) and weakly on T cells, neutrophils and monocytes. Expression of CD37 disappears during B cell development at the plasma cell stage. IPO-24 recognizes the CD37 molecule of about 42-52 kd (4).

References

1) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 544-545. 2) Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 149-151.

NCBI and Uniprot Product Information

• NCBI GI #: 49456709
• NCBI GeneID: 951
• UniProt Accession #: P11049; Q3KPF9; B4DVC1
• Molecular Weight: 22,563 Da
• NCBI Official Full Name: CD37, partial
• NCBI Official Synonym Full Names: CD37 molecule
• NCBI Official Symbol: CD37
• NCBI Official Synonym Symbols: GP52-40; TSPAN26
• NCBI Protein Information: leukocyte antigen CD37; tspan-26; tetraspanin-26; leukocyte surface antigen CD37; cell differentiation antigen 37
• UniProt Protein Name: Leukocyte antigen CD37
• Protein Family: Leukocyte antigen
• UniProt Gene Name: CD37
• UniProt Synonym Gene Names: TSPAN26; Tspan-26
• UniProt Entry Name: CD37_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It may play a role in T-cell-B-cell interactions. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CD37: is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It may play a role in T-cell-B-cell interactions. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: membrane; integral to membrane; immunological synapse

Molecular Function: protein binding

Biological Process: negative regulation of cell proliferation; positive regulation of immunoglobulin production; regulation of humoral immune response; negative regulation of myeloid dendritic cell activation; regulation of defense response to virus; defense response to protozoan