Rabbit anti-Human, Mouse Vacuolar Protein Sorting 53 Polyclonal Antibody | anti-VPS53 antibody

Vacuolar Protein Sorting 53, NT (FLJ10979, HCCS1, hVps53L, MGC39512, pp13624, PP13624, Vacuolar protein sorting-associated protein 53 homolog) (MaxLight 490)

Cat. Num. AAA6505438

• Gene Names: VPS53; HCCS1; PCH2E; hVps53L; pp13624
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Vacuolar Protein Sorting 53; Polyclonal Antibody; NT (FLJ10979; HCCS1; hVps53L; MGC39512; pp13624; PP13624; Vacuolar protein sorting-associated protein 53 homolog) (MaxLight 490); anti-VPS53 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VPS53. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
• Sequence Length: 670

• Applicable Applications for anti-VPS53 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human VPS53 (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of VPS53 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of VPS53 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with VPS53 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-MB468 cells using VPS53 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-VPS53 antibody

Antibodies; Protein Kinases

References

1. Ko, J.K., et.al., J. Cell. Sci. 120(PT 16), 2912-2923 (2007). 2. Zhu, J.D., et.al., Cell Res. 16(9), 780-796 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 21361728
• NCBI GeneID: 55275
• NCBI Accession #: NP_060759
• NCBI GenBank Nucleotide #: NP_060759.2
• UniProt Accession #: Q5VIR6
• Molecular Weight: 79.653
• NCBI Official Full Name: vacuolar protein sorting-associated protein 53 homolog isoform 2
• NCBI Official Synonym Full Names: VPS53 subunit of GARP complex
• NCBI Official Symbol: VPS53
• NCBI Official Synonym Symbols: HCCS1; PCH2E; hVps53L; pp13624
• NCBI Protein Information: vacuolar protein sorting-associated protein 53 homolog
• UniProt Protein Name: Vacuolar protein sorting-associated protein 53 homolog
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS53
• UniProt Entry Name: VPS53_HUMAN

NCBI Description

This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS53: May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Belongs to the VPS53 family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; endosome membrane

Molecular Function: protein binding

Biological Process: protein transport; retrograde transport, endosome to Golgi

Disease: Pontocerebellar Hypoplasia, Type 2e