Mouse anti-Human CD37 Monoclonal Antibody | anti-CD37 antibody
Mouse anti-Human CD37, PE Conjugated mAb
Gene Names
CD37; GP52-40; TSPAN26
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA
Synonyms
CD37; Monoclonal Antibody; Mouse anti-Human CD37; PE Conjugated mAb; anti-CD37 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
4AIPO-24
Form/Format
Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Sequence Length
281
Applicable Applications for anti-CD37 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA (EIA)
Experimental Methods
1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]
2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
Related Product Information for anti-CD37 antibody
4AIPO-24 reacts with CD37 (a.k.a. gp52-40), a 40-52 kDa molecule, which is strongly expressed on B cells from the pre-B cell stage, but not on plasma cells. It is also present at low levels on some T cells, monocytes and granulocytes. CD37 is a stable marker for malignancies derived from mature B cells, such as B-CLL, HCL and all types of B-NHL. CD37 is involved in signal transduction.* CD37 is stable marker for malignancies derived from mature B cells: B-CLL. HCL and B-NHL.
References
1.Kishimoto, T. et al. (1998) Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London. 2.Barclay, A. N. et al. (1993) The Leucocyte Antigen FactsBook, Academic Press Inc., San Diego, California. 3.Wright, MD and Tomlinson, MD (1994) Immunogenetics 15: 588.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,563 Da
NCBI Official Full Name
CD37, partial
NCBI Official Synonym Full Names
CD37 molecule
NCBI Official Symbol
CD37
NCBI Official Synonym Symbols
GP52-40; TSPAN26
NCBI Protein Information
leukocyte antigen CD37
UniProt Protein Name
Leukocyte antigen CD37
Protein Family
UniProt Gene Name
CD37
UniProt Synonym Gene Names
TSPAN26; Tspan-26
NCBI Description
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It may play a role in T-cell-B-cell interactions. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
