Western Blot (WB)
(Western blot analysis of lysate from mouse liver tissue, using Egfr Antibody (P1116). MBS9204428 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Rabbit anti-Mouse Egfr Polyclonal Antibody | anti-Egfr antibody
Mouse Egfr Antibody (P1116)
Western Blot (WB)
(Western blot analysis of lysate from mouse liver tissue, using Egfr Antibody (P1116). MBS9204428 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(Western blot analysis of lysate from mouse liver tissue, using Egfr Antibody (P1116). MBS9204428 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Paria B.C.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:55-59(1993).
Hibbs M.L.,et al.Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases.
Luetteke N.C.,et al.Genes Dev. 8:399-413(1994).
Avivi A.,et al.Oncogene 6:673-676(1991).
NCBI and Uniprot Product Information
Uniprot Description
EGFR: a receptor tyrosine kinase. This is a receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor. EGFR is involved in the control of cell growth and differentiation. It is a single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation. It can be phosphorylated and activated by Src. Activated EGFR binds the SH2 domain of phospholipase C-gamma (PLC-gamma), activating PLC-gamma-mediated downstream signaling. Phosphorylated EGFR binds Cbl, leading to its ubiquitination and degradation. Grb2 and SHC bind to phospho-EGFR and are involved in the activation of MAP kinase signaling pathways. Phosphorylation on Ser and Thr residues is thought to represent a mechanism for attenuation of EGFR kinase activity. EGFR is overexpressed in breast, head and neck cancers, correlating with poor survival. Activating somatic mutations are seen in lung cancer, corresponding to the minority of patients with strong responses to the EGFR inhibitor Iressa (gefitinib). Mutations and amplifications are also seen in glioblastoma, and upregulation is seen in colon cancer and neoplasms. In xenografts, inhibitors synergize with cytotoxic drugs in the inhibition of many tumor types. Inhibitors include: Iressa/ZD1839, Erbitux, Tarceva, and lapatinib. Four alternatively spliced isoforms have been described.
Protein type: Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; Tumor suppressor; Kinase, protein; EC 2.7.10.1; TK group; EGFR family
Cellular Component: Golgi apparatus; focal adhesion; cell surface; basolateral plasma membrane; endoplasmic reticulum; integral to membrane; lipid raft; membrane; perinuclear region of cytoplasm; endocytic vesicle; apical plasma membrane; cytoplasm; plasma membrane; endosome membrane; intracellular; nucleus; endosome; receptor complex
Molecular Function: identical protein binding; epidermal growth factor receptor activity; epidermal growth factor binding; nitric-oxide synthase regulator activity; protein phosphatase binding; actin filament binding; integrin binding; signal transducer activity; enzyme binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; ATP binding; receptor binding; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase binding; protein kinase activity; transferase activity; protein binding; transmembrane receptor activity; protein heterodimerization activity; ubiquitin protein ligase binding; kinase activity; chromatin binding; glycoprotein binding
Biological Process: epidermis development; embryonic placenta development; positive regulation of nitric oxide biosynthetic process; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; translation; positive regulation of smooth muscle cell proliferation; multicellular organismal development; protein amino acid autophosphorylation; cell morphogenesis; positive regulation of vasodilation; signal transduction; positive regulation of synaptic transmission, glutamatergic; protein amino acid phosphorylation; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; cell-cell adhesion; ovulation cycle; cell surface receptor linked signal transduction; learning and/or memory; hair follicle development; positive regulation of superoxide release; salivary gland morphogenesis; positive regulation of cell proliferation; negative regulation of mitotic cell cycle; regulation of peptidyl-tyrosine phosphorylation; epidermal growth factor receptor signaling pathway; positive regulation of DNA repair; digestive tract morphogenesis; regulation of cell proliferation; cell proliferation; positive regulation of protein kinase B signaling cascade; cerebral cortex cell migration; positive regulation of vasoconstriction; positive regulation of transcription from RNA polymerase II promoter; regulation of nitric-oxide synthase activity; positive regulation of protein amino acid phosphorylation; astrocyte activation; negative regulation of protein catabolic process; response to calcium ion; phosphorylation; neurite morphogenesis; positive regulation of phosphorylation; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of cell migration; positive regulation of inflammatory response
Research Articles on Egfr
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Product Notes
The Egfr egfr (Catalog #AAA9204428) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Egfr Antibody (P1116) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Egfr can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Egfr egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Egfr, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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