Rabbit anti-Human, mouse Elongin A Polyclonal Antibody | anti-TCEB3 antibody

Elongin A Antibody (N-term)

Cat. Num. AAA9207620

• Gene Names: TCEB3; EloA; SIII; TCEB3A; SIII p110
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Elongin A; Polyclonal Antibody; Elongin A Antibody (N-term); Transcription elongation factor B polypeptide 3; Elongin 110 kDa subunit; Elongin-A; EloA; RNA polymerase II transcription factor SIII subunit A1; SIII p110; TCEB3; anti-TCEB3 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Elongin A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-127 amino acids from the N-terminal region of human Elongin A.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.31 (varies by lot)
• Sequence Positions: 97-127
• Sequence Length: 798

• Applicable Applications for anti-TCEB3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Precautions: Elongin A Antibody (N-term) is for research use only
• Function: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of Elongin A (arrow) using rabbit polyclonal Elongin A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Elongin A gene.)

Western Blot (WB)

(Elongin A Antibody western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Elongin A antibody detected the Elongin A protein (arrow).)

Related Product Information for anti-TCEB3 antibody

Elongin A is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

References

Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004).
Tamura, K., et al., Biochem. Biophys. Res. Commun. 309(1):189-195 (2003).
Kile, B.T., et al., Trends Biochem. Sci. 27(5):235-241 (2002).
Yamazaki, K., et al., J. Biol. Chem. 277(29):26444-26451 (2002).
Kamura, T., et al., J. Biol. Chem. 276(32):29748-29753 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 208022641
• NCBI GeneID: 6924
• NCBI Accession #: NP_003189.2
• NCBI GenBank Nucleotide #: NM_003198.2
• UniProt Accession #: Q14241; Q8IXH1; B2R7Q8
• Molecular Weight: 89909
• NCBI Official Full Name: transcription elongation factor B polypeptide 3
• NCBI Official Synonym Full Names: transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
• NCBI Official Symbol: TCEB3
• NCBI Official Synonym Symbols: EloA; SIII; TCEB3A; SIII p110
• NCBI Protein Information: transcription elongation factor B polypeptide 3
• UniProt Protein Name: Transcription elongation factor B polypeptide 3
• Protein Family: Transcription elongation factor B polypeptide
• UniProt Gene Name: TCEB3
• UniProt Synonym Gene Names: EloA
• UniProt Entry Name: ELOA1_HUMAN

NCBI Description

This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. [provided by RefSeq, Jul 2008]

Uniprot Description

TCEB3: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). Heterotrimer of an A (A1, A2 or A3), B and C subunit. The C subunit mediates the binding of the elongin BC complex to subunit A.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1p36.1

Cellular Component: nucleoplasm; extracellular space; cytoplasm; integral to membrane; nucleus

Molecular Function: DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression