Mouse CD3 Monoclonal Antibody | anti-CD3E antibody

Mouse anti Bovine CD3

Cat. Num. AAA225383

• Reactivity: Bovine, Bison, Water Buffalo
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry
• Purity: Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
• Synonyms: CD3; Monoclonal Antibody; Mouse anti Bovine CD3; CD3 Antibody; anti-CD3E antibody

• Host: Mouse
• Reactivity: Bovine, Bison, Water Buffalo
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: MM1A
• Purity/Purification: Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
• Form/Format: Liquid
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 192

• Applicable Applications for anti-CD3E antibody: Flow Cytometry (FC/FACS), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: Neat-1/10
• Immunogen: Monocyte enriched bovine mononuclear cells
• Buffer Solution: Serum free tissue culture medium containing proprietary protein free supplement with 0.09% Sodium Azide (NaN3)
• Preparation and Storage: Store at +4 degree C. DO NOT FREEZE.; Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Flow Cytometry (FC/FACS)

(Flow cytometric staining of bovine peripheral blood lymphocytes.A. Mouse IgG1 negative control followed by Goat anti Mouse IgG1:PE.B. Mouse anti Bovine CD3 antibody, clone MM1A followed by Goat anti Mouse IgG1:PE secondary antibody |.Both the Mouse IgG1 negative control and Mouse anti Bovine CD3 are plotted against bovine CD4 as recognized by FITC conjugated Mouse anti Bovine CD4 antibody, clone CC8.)

Related Product Information for anti-CD3E antibody

Mouse anti Bovine CD3, clone MM1A, recognizes bovine CD3. CD3 plays an important role in T cell activation and in expression of intact TCR molecules. Clone MM1A detects bovine CD3 and reacts with bovine alpha beta and gamma delta TCR+ T cells and recognizes an epitope located on the epsilon chain of CD3 (MacHugh et al. 1998). Mouse anti Bovine CD3, clone MM1A also reacts with Zebu (Conrad et al. 2007).

NCBI and Uniprot Product Information

• NCBI GI #: 116256283
• NCBI GeneID: 281054
• UniProt Accession #: Q28073; Q3ZCH2
• Molecular Weight: 21,523 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule
• NCBI Official Symbol: CD3E
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Protein Family: CD302 antigen
• UniProt Gene Name: CD3E

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.