Flow Cytometry (FC/FACS)
(Flow cytometric staining of bovine peripheral blood lymphocytes.A. Mouse IgG1 negative control followed by Goat anti Mouse IgG1:PE.B. Mouse anti Bovine CD18 antibody, clone BAQ30A followed by Goat anti Mouse IgG1:PE secondary antibody |.Both the Mouse IgG1 negative control and Mouse anti Bovine CD18 are plotted against bovine CD4 as recognized by FITC conjugated Mouse anti Bovine CD4 antibody, clone CC8 .)
Mouse CD18 Monoclonal Antibody | anti-ITGB2 antibody
Mouse anti Bovine CD18
Gene Names
ITGB2; CD18
Reactivity
Bovine, Goat, Sheep, Bison, Water Buffalo, Cat, Dog, Alpaca, Llama, Human
Applications
Flow Cytometry, Functional Assay, Functional Assay
Purity
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Synonyms
CD18; Monoclonal Antibody; Mouse anti Bovine CD18; CD18 Antibody; anti-ITGB2 antibody
Host
Mouse
Reactivity
Bovine, Goat, Sheep, Bison, Water Buffalo, Cat, Dog, Alpaca, Llama, Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
BAQ30A
Purity/Purification
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Form/Format
Liquid
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
769
Applicable Applications for anti-ITGB2 antibody
Flow Cytometry (FC/FACS), Functional Assays (FN)
Application Notes
FC/FACS: Neat-1/10
Immunogen
Cells from multiple species
Buffer Solution
Serum free tissue culture medium containing proprietary protein free supplement with 0.09% Sodium Azide (NaN3)
Preparation and Storage
Store at +4 degree C. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Flow Cytometry (FC/FACS)
(Flow cytometric staining of bovine peripheral blood lymphocytes.A. Mouse IgG1 negative control followed by Goat anti Mouse IgG1:PE.B. Mouse anti Bovine CD18 antibody, clone BAQ30A followed by Goat anti Mouse IgG1:PE secondary antibody |.Both the Mouse IgG1 negative control and Mouse anti Bovine CD18 are plotted against bovine CD4 as recognized by FITC conjugated Mouse anti Bovine CD4 antibody, clone CC8 .)
Flow Cytometry (FC/FACS)
(Flow cytometric staining of bovine peripheral blood lymphocytes.A. Mouse IgG1 negative control followed by Goat anti Mouse IgG1:PE.B. Mouse anti Bovine CD18 antibody, clone BAQ30A followed by Goat anti Mouse IgG1:PE secondary antibody |.Both the Mouse IgG1 negative control and Mouse anti Bovine CD18 are plotted against bovine CD4 as recognized by FITC conjugated Mouse anti Bovine CD4 antibody, clone CC8 .)
Related Product Information for anti-ITGB2 antibody
Mouse anti Bovine CD18, clone BAQ30A, recognizes bovine CD18 also known as ITGB2 or integrin beta-2. CD18 is a receptor for ICAM1, ICAM2, ICAM3, and ICAM4. It recognizes the G-P-R motif in the fibrinogen alpha chain and is involved in leukocyte adhesion as well as transmigration of T cells and neutrophils.
Different from other organisms, the signal peptide of ruminant CD18, the beta subunit, is not cleaved and remains intact on the mature CD18 molecule, leukotoxin from Pasteurella haemolytica binds to the intact signal peptide on ruminant leukocytes causing acute inflammation and lung damage (Shanthalingam et al. 2016). Holstein cattle harbor a mutation in CD18 causing bovine leukocyte adhesion deficiency (BLAD) syndrome (Kehrli et al. 1992) with decreased expression of beta 2 integrins and lowered susceptibility to cytolysis in Pasteurella infections (Jeyaseelan et al. 2000).
Different from other organisms, the signal peptide of ruminant CD18, the beta subunit, is not cleaved and remains intact on the mature CD18 molecule, leukotoxin from Pasteurella haemolytica binds to the intact signal peptide on ruminant leukocytes causing acute inflammation and lung damage (Shanthalingam et al. 2016). Holstein cattle harbor a mutation in CD18 causing bovine leukocyte adhesion deficiency (BLAD) syndrome (Kehrli et al. 1992) with decreased expression of beta 2 integrins and lowered susceptibility to cytolysis in Pasteurella infections (Jeyaseelan et al. 2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
84,400 Da
NCBI Official Full Name
Integrin beta-2
NCBI Official Synonym Full Names
integrin subunit beta 2
NCBI Official Symbol
ITGB2
NCBI Official Synonym Symbols
CD18
NCBI Protein Information
integrin beta-2
UniProt Protein Name
Integrin beta-2
Protein Family
UniProt Gene Name
ITGB2
UniProt Synonym Gene Names
CD18
Uniprot Description
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. Integrin alpha-L/beta-2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.
Research Articles on ITGB2
Similar Products
Product Notes
The ITGB2 itgb2 (Catalog #AAA225389) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Bovine CD18 reacts with Bovine, Goat, Sheep, Bison, Water Buffalo, Cat, Dog, Alpaca, Llama, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD18 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Functional Assays (FN). FC/FACS: Neat-1/10. Researchers should empirically determine the suitability of the ITGB2 itgb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD18, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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