Mouse anti-Human TCR Gamma/Delta Monoclonal Antibody | anti-CD3E antibody

Mouse Anti Human TCR Gamma/Delta: FITC

Cat. Num. AAA225831

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: >95% by SDS PAGE; Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Synonyms: TCR Gamma/Delta; Monoclonal Antibody; Mouse Anti Human TCR Gamma/Delta: FITC; TCR Gamma/Delta antibody; B1; anti-CD3E antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: B1
• Purity/Purification: >95% by SDS PAGE; Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Form/Format: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - Liquid

• Applicable Applications for anti-CD3E antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: Max. Dilution: Neat; Use 4ul of the suggested working dilution to label 1x106 cells in 100ul
• Preservative Stabilizers: <0.1% Sodium Azide (NaN3) 0.2% Bovine Serum Albumin
• Buffer Solution: Phosphate Buffered Saline
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE.; This product should be stored undiluted.; This product is photosensitive and should be protected from light.

Testing Data

(Surface Staining of human peripheral blood lymphocytes with Mouse anti Human TCR gamma/delta:FITC )

Testing Data

(Surface Staining of human peripheral blood lymphocytes with Mouse anti Human TCR gamma/delta:RPE )

Related Product Information for anti-CD3E antibody

Mouse anti Human TCR gamma/delta, clone B1 recognizes TCR gamma/delta, a T cell receptor expressed by a small subset of T cells. In healthy individuals these TCR gamma/delta cells account for 1-10% of peripheral blood lymphocytes (Gomes et al. 2010).
TCR gamma/delta is composed of disulfide bonded gamma and delta chains that can bind directly to pathogen derived glycoprotein's or non-classical MHC molecules on tumor cells (Kuhns et al. 2006).
This clone B1 has been used as a blocking antibody in experiments to examine the recognition of tumor cells by TCR gamma/delta cells. These experiments supported the hypothesis that innate NK receptors on gamma/delta cells can also bind and recognize tumor cells (Correia et al. 2011).

NCBI and Uniprot Product Information

• NCBI GI #: 1345708
• NCBI GeneID: 916
• UniProt Accession #: P07766
• NCBI Official Synonym Full Names: CD3e molecule
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN

NCBI Description

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; SH3 domain binding; protein kinase binding

Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of interleukin-4 production; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; protein complex assembly; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; positive regulation of T cell anergy; transmembrane receptor protein tyrosine kinase signaling pathway; response to nutrient

Disease: Immunodeficiency 18