Mouse CD172a Monoclonal Antibody | anti-SIRPA antibody

Mouse anti Bovine CD172a

Cat. Num. AAA225382

• Gene Names: SIRPA; MYD-1; PTPNS1
• Reactivity: Bovine, Bison, Water Buffalo, Sheep, Horse, Dog, Cat
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
• Synonyms: CD172a; Monoclonal Antibody; Mouse anti Bovine CD172a; CD172a Antibody; anti-SIRPA antibody

• Host: Mouse
• Reactivity: Bovine, Bison, Water Buffalo, Sheep, Horse, Dog, Cat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: DH59B
• Purity/Purification: Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
• Form/Format: Liquid
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 506

• Applicable Applications for anti-SIRPA antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin
• Application Notes: FC/FACS: Neat-1/10
• Immunogen: Cells from multiple species with the final screening of the fusion on cells from dog
• Buffer Solution: Serum free tissue culture medium containing proprietary protein free supplement with 0.09% Sodium Azide (NaN3)
• Preparation and Storage: Store at +4 degree C. DO NOT FREEZE.; Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Flow Cytometry (FC/FACS)

(Flow cytometric staining of bovine peripheral blood monocytes.A. Mouse IgG1 negative control followed by Goat anti Mouse IgG1:PE Mouse anti Bovine CD172a antibody, clone DH59B followed by Goat anti Mouse IgG1:PE secondary antibody |.Both the Mouse IgG1 negative control and Mouse anti Bovine CD172a are plotted against bovine CD11b as recognized by FITC conjugated Mouse anti Bovine CD11b antibody, clone CC126.)

Related Product Information for anti-SIRPA antibody

Mouse anti Bovine CD172a, clone DH59B, recognizes bovine CD172a also known as SIRP alpha or MyD-1 antigen. CD172a is a transmembrane signal regulatory protein expressed primarily by macrophages, monocytes, dendritic cells, granulocytes, myeloid progenitors, hematopoietic stem cells, and neurons (Barclay et al. 2006). The extracellular region of SIRP family consists of three immunoglobulin superfamily (IgSF) domains; two IgC and one IgV domain (Barclay et al. 2006).

The IgV domain of CD172a binds to CD47 (Hatherley et al. 2007).The binding domain of CD172a is analogous to that of immunoglobulins and T cell receptors and is involved in myeloid cell activation (Berg et al.2004). However, signaling via CD172a is mainly inhibitory to cell function and phagocytosis (Oldenborg et al. 2001). CD172a expressing cells are stimulated in Mycobacterium tuberculosis infection, influencing migration of dendritic cells and macrophages, phagocytosis, and granuloma formation (Waters et al. 2009).

NCBI and Uniprot Product Information

• NCBI GI #: 29427567
• NCBI GeneID: 327666
• UniProt Accession #: O46631; O46632
• Molecular Weight: 55,093 Da
• NCBI Official Full Name: Tyrosine-protein phosphatase non-receptor type substrate 1
• NCBI Official Symbol: SIRPA
• NCBI Official Synonym Symbols: MYD-1; PTPNS1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type substrate 1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type substrate 1
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: SIRPA
• UniProt Synonym Gene Names: MYD1; PTPNS1; SHPS1; SIRP; SHP substrate 1; SHPS-1; Sirp-alpha-1

Uniprot Description

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells ().