Mouse anti-Human CD3 Monoclonal Antibody | anti-CD3 antibody

Mouse anti Human CD3:FITC

Cat. Num. AAA226112

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human
• Applications: ELISA
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant; >90 % by SDS PAGE
• Synonyms: CD3; Monoclonal Antibody; Mouse anti Human CD3:FITC; CD3 antibody | OKT3; anti-CD3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: OKT3
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant; >90 % by SDS PAGE
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.2mg/ml (varies by lot)

• Applicable Applications for anti-CD3 antibody: ELISA (EIA)
• Application Notes: FC/FACS: Neat
• Immunogen: Human peripheral blood lymphocytes
• Conjugation: FITC
• Preparation and Storage: Store at +4 degree C. DO NOT FREEZE.; This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Testing Data

(Surface staining of human peripheral blood lymphocytes with Mouse anti Human CD3:FITC (MBS226112) (Blue) with a relevant isotype control (Red))

Testing Data

(Surface staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE (Blue) with a relevant isotype control (Grey))

Related Product Information for anti-CD3 antibody

Mouse anti Human CD3 antibody, clone OKT3, recognizes T-cell surface glycoprotein CD3 epsilon chain (CD3epsilon), also known as T-cell surface antigen T3/Leu-4 epsilon. CD3epsilon is a subunit within the multiprotein cell surface receptor TCR-CD3 complex, which is expressed on the surface of mature T cells, NKT cells and thymocytes (Mariuzza et al. 2020). The CD3 complex is required for intracellular signaling and for TCR surface expression. Recruitment of the TCR/CD3 complex initiates early events leading to proliferation and differentiation of mature T cells into an effector cell (Guy & Vignali 2009).

Mice that lack the CD3epsilon gene exhibit early arrest in T cell development and mutation of the gene in humans has been seen to result in severe combined immunodeficiency (SCID) (DeJarnette et al. 1998, de Saint Basile et al. 2004).

Mouse anti Human CD3, clone OKT3 is commonly used in flow cytometry in the phenotyping human T cells. Characterization of the OKT3 clone has shown that it is able to compete and block the binding of the CD3 clones SK7 and UCHT1 to human T cells (Li et al. 2005). The OKT3 clone has also been found to have immunosuppressive properties in vivo. As result of this, the clone was humanized to be used as a therapeutic drug called Muromonomab (Orthoclone). Muromonomab was used as an immunosuppressive including for after solid organ transplants, to prevent the activation of CD4- and CD8- T cells (Wilde & Goa 1996). However, the drug has since been discontinued due to the development of other treatments with similar efficacy and fewer side effects (Reichert 2012).

NCBI and Uniprot Product Information

• NCBI GI #: 1345708
• NCBI GeneID: 916
• UniProt Accession #: P07766; A8K997
• Molecular Weight: 207
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule, epsilon (CD3-TCR complex)
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Protein Family: CD302 antigen
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN

NCBI Description

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; SH3 domain binding; protein kinase binding

Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of interleukin-4 production; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; protein complex assembly; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; positive regulation of T cell anergy; transmembrane receptor protein tyrosine kinase signaling pathway; response to nutrient

Disease: Immunodeficiency 18