Rat anti-Human CD3 Monoclonal Antibody | anti-CD3 antibody

RAT ANTI HUMAN CD3

Cat. Num. AAA215051

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human
• Applications: Flow Cytometry, Immunohistochemistry, Western Blot, Immunofluorescence
• Synonyms: CD3; Monoclonal Antibody; RAT ANTI HUMAN CD3; anti-CD3 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: CD3-12
• Specificity: Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).
• Form/Format: Purified IgG - liquid

• Applicable Applications for anti-CD3 antibody: Flow Cytometry (FC), Immunohistochemistry - Frozen/Paraffin (IHC-F/P), Western Blotting (WB), Immunofluorescence (IF)
• Application Notes: FC: 1/50 - 1/100 (1); IHC - Frozen: 1/100; IHC - Paraffin: 1/100 (2); ; (1) Membrane permeabilization is required for this application. The use of Leucoperm (MBS225936) is recommended for this purpose.; (2) This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose. ; ; Optimal dilutions/concentrations should be determined by the end user.; Other applications not yet tested, however should not be excluded.
• Species Cross Reactivity: Bovine, Dog, Horse, Rhesus Monkey, Pig, Chicken, Mouse, Duck, Koala, Harbour Porpoise, Alpaca, Cynomolgus monkey, Spotted Hyena, Sea Lion, Cat, Amazon Parrot, Raccoon, Great horned owl (Bubo virginianus), Bullfrog, Xenopus, Rabbit; Based on sequence similarity, is expected to react with: Mammals, Birds, Amphibia; N.B. Antibody reactivity and working conditions may vary between species.
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Buffer Solution: Phosphate buffered saline
• Preservative Stabilizers: 0.09% Sodium Azide
• Carrier Free: Yes
• Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
• Immunogen: Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC)
• Preparation and Storage: It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 4502671
• NCBI GeneID: 916
• NCBI Accession #: NP_000724.1
• NCBI GenBank Nucleotide #: NM_000733.3
• UniProt Accession #: P07766; A8K997
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule, epsilon (CD3-TCR complex)
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Protein Family: CD302 antigen
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN

NCBI Description

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; protein kinase binding; SH3 domain binding

Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; T cell receptor signaling pathway; negative thymic T cell selection; positive regulation of interleukin-4 production; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; protein complex assembly; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; positive regulation of T cell anergy; response to nutrient; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Immunodeficiency 18