Rabbit anti-Human CD28 Monoclonal Antibody | anti-CD28 antibody

Anti-CD28 antibody (DM63), Rabbit mAb

Cat. Num. AAA188198

• Gene Names: CD28; Tp44
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay
• Purity: Purified from cell culture supernatant by affinity chromatography
• Synonyms: CD28; Monoclonal Antibody; Anti-CD28 antibody (DM63); Rabbit mAb; Tp44; anti-CD28 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: DM61
• Purity/Purification: Purified from cell culture supernatant by affinity chromatography
• Form/Format: Liquid. 50% Glycerol; PBS, pH7.4; 0.1% BSA; 0.1% Procline 300

• Applicable Applications for anti-CD28 antibody: ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: ELISA: 1:5000-10,000; FC/FACS: 1:100
• Immunogen: Recombinant human CD28 (Asn19-Pro152) produced by using human HEK293 cells
• Reconstitution: Reconstitute with deionized water
• Preparation and Storage: Store at -20 degree C for 12 months (Avoid repeated freezing and thawing)

ELISA (EIA)

(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD28 protein, mFc-His tagged protein can bind Rabbit anti-CD28 monoclonal antibody (clone: DM63) in a linear range of 1-100 ng/ml.)

Testing Data

(Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CD28 (B) were surface stained with Rabbit anti-CD28 monoclonal antibody 1ug/ml (clone: DM63) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.)

Flow Cytometry (FC/FACS)

(Figure 3. Flow cytometry data of serially titrated Rabbit anti-CD28 monoclonal antibody (clone: DM63) on Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Testing Data

(Figure 4. Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Related Product Information for anti-CD28 antibody

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NM_001243077.1
• UniProt Accession #: P10747; Q13964; Q52M23; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
• Molecular Weight: 25,066 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; CD28 antigen
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN

NCBI Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; plasma membrane; immunological synapse; cytosol; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; protease binding; coreceptor activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of translation; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine biosynthetic process; T cell receptor signaling pathway; positive regulation of inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; positive regulation of T cell proliferation; regulatory T cell differentiation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of viral genome replication; positive regulation of mitosis; negative thymic T cell selection; regulation of defense response to virus by virus; humoral immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; T cell costimulation; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of transcription from RNA polymerase II promoter