Rabbit anti-Mouse TREM-1 Polyclonal Antibody | anti-TREM1 antibody

Anti-TREM-1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101919

• Reactivity: Mouse
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: TREM-1; Polyclonal Antibody; Anti-TREM-1 Antibody; Rabbit Polyclonal; TREM-1/TREM1 Antibody; Rabbit PAb; Antigen Affinity Purified; triggering receptor expressed on myeloid cells 1; anti-TREM1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse TREM-1/TREM1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TREM1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse TREM-1/TREM1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse TREM-1/TREM1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse TREM1 in mouse liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of TREM1 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human TREM1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Related Product Information for anti-TREM1 antibody

TREM1 (triggering receptor expressed on myeloid cells) is a type I transmembrane protein with a single Ig-like domain, and is selectively expressed on blood neutrophils and a subset of monocytes. As a member of the growing family of receptors related to NK cell receptors, TREM1 activates downstream signaling events with the help of an adapter protein called DAP12. Expression of TREM1 is up-regulated by bacterial LPS, a ligand for TLR4, as well as lipoteichoic acid. Although its natural ligand has not been identified, engagement of TREM1 with agonist mAbs triggers secretion of the proinflammatory cytokines TNF-alpha and IL-1beta, as well as chemokines such as IL-8 and monocyte chemoattractant protein (MCP)-1. Intracellularly, TREM1 induces Ca2+ mobilization and tyrosine phosphorylation of extracellular signal-related kinase 1 (ERK1), ERK2 and phospholipase C-gamma. In an animal model of LPS-induced septic shock, blockade of TREM1 signaling inhibited hyperresponsiveness and death. Thus, it has been demonstrated that TREM1 performs a critical function in immune responses involved in host defense against microbial challenges, and is suggested to be a potential therapeutic target for septic shock.

References

Bouchon, A. et al., 2000, J. Immunol. 164: 4991-4995. Bouchon, A. et al., 2001, Nature. 410: 1103-1107. Bleharski, J.R. et al., 2003, J. Immunol. 170: 3812-3818.

NCBI and Uniprot Product Information

• NCBI GI #: 81175033
• NCBI GeneID: 58217
• UniProt Accession #: Q9JKE2; Q3TAL7
• Molecular Weight: 25,409 Da
• NCBI Official Full Name: Triggering receptor expressed on myeloid cells 1
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 1
• NCBI Official Symbol: Trem1
• NCBI Protein Information: triggering receptor expressed on myeloid cells 1; triggering receptor expressed in monocytes 1
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 1
• Protein Family: Triggering receptor expressed on myeloid cells
• UniProt Gene Name: Trem1
• UniProt Synonym Gene Names: TREM-1
• UniProt Entry Name: TREM1_MOUSE

Uniprot Description

TREM1: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: membrane; cell; integral to membrane

Biological Process: neutrophil chemotaxis; cell migration; cytokine metabolic process; cytokine secretion during immune response; chemokine metabolic process