Mouse anti-Sheep CD25 Monoclonal Antibody | anti-CD25 antibody

MOUSE ANTI SHEEP CD25

Cat. Num. AAA224836

• Reactivity: Sheep
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CD25; Monoclonal Antibody; MOUSE ANTI SHEEP CD25; anti-CD25 antibody

• Host: Mouse
• Reactivity: Sheep
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9.14
• Specificity: CD25
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 275

• Applicable Applications for anti-CD25 antibody: Immunohistochemistry (IHC) Formalin, Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: 1/10; 1/50
• Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Preservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Buffer Solution: Phosphate buffered saline
• Preparation and Storage: Store at +4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. ; Shelf Life: 18 months from date of despatch

Flow Cytometry (FC/FACS)

(Staining of ovine peripheral blood lymphocytes with Mouse anti Sheep CD25 followed by Goat anti Mouse IgG (H/L):FITC . Ovine blood was stimulated for 48 hours with 10 ug/ml LPS and 10 ug/ml PHA prior to staining)

Related Product Information for anti-CD25 antibody

Mouse anti sheep CD25, clone 9.14, is specific for the ovine homologue of human CD25, also known as interleukin-2 receptor alpha chain (IL-2Ralpha), a single pass type I membrane protein expressed by activated ovine T-cells. Ovine Interleukin-2 is a cytokine involved in the proliferation, growth and differentiation of T-cells, B-cells and NK cells and its receptor is composed of 3 subunits, an alpha chain (CD25), a beta chain (CD122) and a gamma chain (CD132). A non-covalent association of the alpha and beta subunits is required to form the high affinity receptor for IL-2. Immunoprecipitation studies have demonstrated that clone 9.14 precipitates a band of approximately 47 kDa under reducing conditions, which is that expected of the mature protein due to high glycosylation, and is consistent with the observed molecular weight of IL-2Ralpha in other species such as humans (Verhagen, A. M., et. al.1993). Research has demonstrated that antibodies to CD4, FoxP3 and CD25 may be used in studies of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity (Rocchi, M.S,. et. al 2011).

NCBI and Uniprot Product Information

• NCBI GI #: 124320
• NCBI GeneID: 443435
• UniProt Accession #: P26898
• Molecular Weight: 30,904 Da
• NCBI Official Full Name: Interleukin-2 receptor subunit alpha
• NCBI Official Symbol: IL2RA
• NCBI Protein Information: interleukin-2 receptor subunit alpha
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• UniProt Gene Name: IL2RA
• UniProt Synonym Gene Names: IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_SHEEP

Uniprot Description

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.