Mouse anti-Human CD200 Monoclonal Antibody | anti-CD200 antibody

Anti-Hu CD200 Alexa Fluor647

• Gene Names: CD200; MRC; MOX1; MOX2; OX-2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD200; Monoclonal Antibody; Anti-Hu CD200 Alexa Fluor647; MRC; MOX; OX-2; CD200 molecule; anti-CD200 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: OX-104
• Specificity: The mouse monoclonal antibody OX-104 recognizes an extracellular epitope of CD200, a type-1 glycoprotein of the immunoglobulin superfamily, which is expressed in neurons, B and T cell subsets, keratinocytes, follicular dendritic cells, and ovarian cells.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD200 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: Alexa Fluor647
• Immunogen: recombinant human CD200
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD200 (OX-104) Alexa Fluor 647 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD200 (OX-104) Alexa Fluor 647 antibody (4 ul reagent / 100 ul of peripheral whole blood) and anti-human CD3 (UCHT1) PE antibody (20 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human CD200 positive CD3 negative lymphocytes (red-filled) from CD200 negative T cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD200 (OX-104) Alexa Fluor 647 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD200 antibody

CD200 (also known as OX2 or MRC) is a type-1 membrane glycoprotein, which contains two extracellular immunoglobulin domains, transmembrane domain and cytoplasmic domain. It is expressed by neuronal cells, B and T cell subsets, follicular dendritic cells, keratinocytes, and ovarian cells. The interaction between CD200 and its receptor CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF-alpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes. It seems CD200 is also involved in maternal tolerance and its decreased expression in hair follicle correlates with follicular miniaturization.

References

*Garza LA, Yang CC, Zhao T, Blatt HB, Lee M, He H, Stanton DC, Carrasco L, Spiegel JH, Tobias JW, Cotsarelis G: Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells. J Clin Invest. 2011 Feb;121(2):613-22, URL: http://www.ncbi.nlm.nih.gov/pubmed/21206086,*Li Y, Zhao LD, Tong LS, Qian SN, Ren Y, Zhang L, Ding X, Chen Y, Wang YX, Zhang W, Zeng XF, Zhang FC, Tang FL, Zhang X, Ba DN, He W, Cao XT, Lipsky PE: Aberrant CD200/CD200R1 expression and Functional Application (FUNC)tion in systemic lupus erythematosus contributes to abnormal T-cell responsiveness and dendritic cell activity. Arthritis Res Ther. 2012 May 23;14(3):R123. doi: 10.1186/ar3853., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=Li+Y%2C+,*Sopp P, Werling D, Baldwin C: Sopp P, Werling D, Baldwin C: Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):106-14, URL: http://www.ncbi.nlm.nih.gov/pubmed/term=sopp+p+we,*Shiratori I, Yamaguchi M, Suzukawa M, Yamamoto K, Lanier LL, Saito T, Arase H: Down-regulation of basophil Functional Application (FUNC)tion by human CD200 and human herpesvirus-8 CD200. J Immunol. 2005 Oct 1;175(7):4441-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=shiratori,*Colmont CS, Benketah A, Reed SH, Hawk NV, Telford WG, Ohyama M, Udey MC, Yee CL, Vogel JC, Patel GK: CD200-expressing human basal cell carcinoma cells initiate tumor growth. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1434-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=colmont+c,*Foster-Cuevas M, Wright GJ, Puklavec MJ, Brown MH, Barclay AN: Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor. J Virol. 2004 Jul;78(14):7667-76., URL: http://www.ncbi.nlm.nih.gov/pubmed/15220441,*Meuth SG, Simon OJ, Grimm A, Melzer N, Herrmann AM, Spitzer P, Landgraf P, Wiendl H: CNS inflammation and neuronal degeneration is aggravated by impaired CD200-CD200R-mediated macrophage silencing. J Neuroimmunol. 2008 Feb;194(1-2):62-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/18164423

NCBI and Uniprot Product Information

• NCBI GI #: 311033489
• NCBI GeneID: 4345
• UniProt Accession #: P41217; Q6J2Q6; Q6PIQ4; Q8TB85; Q9H3J3; B3KQI1; B4DLW9; D3DN65
• Molecular Weight: 32,975 Da
• NCBI Official Full Name: OX-2 membrane glycoprotein
• NCBI Official Synonym Full Names: CD200 molecule
• NCBI Official Symbol: CD200
• NCBI Official Synonym Symbols: MRC; MOX1; MOX2; OX-2
• NCBI Protein Information: OX-2 membrane glycoprotein
• UniProt Protein Name: OX-2 membrane glycoprotein
• Protein Family: OX-2 membrane glycoprotein
• UniProt Gene Name: CD200
• UniProt Synonym Gene Names: MOX1; MOX2

NCBI Description

This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.