Human CD200 ELISA Kit | CD200 elisa kit

Human CD200 Quick ELISA Kit

Cat. Num. AAA1753945

• Gene Names: CD200; MRC; MOX1; MOX2; OX-2
• Reactivity: Human
• Synonyms: CD200; Human CD200 Quick ELISA Kit; CD200/OX2|antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2; CD200 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CD200

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 31.2pg/ml-2,000pg/ml
• Sensitivity: <10pg/ml
• Capture/Detection Antibodies: Capture antibody- monoclonal antibody from mouse|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.015|0.062|0.099|0.185|0.329|0.625|1.179|2.046
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|89|252|897|; Standard deviation|4.36|16.12|69.06|; CV(%)|4.9%|6.4%|7.7%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|75|251|960; Standard deviation|4.12|14.30|72; CV(%)|5.5%|5.7%|7.5%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 89|75|93|98|88|4.66|5.3%; Sample 2: 252|245|216|254|241|7.47|3.1%; Sample 3: 897|862|897|871|881|22.02|2.5%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q31-G232
• Anticoagulant: heparin or EDTA
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human CD200 with <10pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human CD200 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD200 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for CD200. The detection antibody is a HRP linked goat polyclonal antibody processed by a proprietary method. The kit contains recombinant Human CD200 with immunogen: Expression system for standard: NS0; Immunogen sequence: Q31-G232. The kit is analytically validated with ready to use reagents.; ; To measure Human CD200, add standards and samples to the wells, then add HRP linked detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CD200 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD200 in the sample.
• Assay Procedure Step 1: 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.25pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for CD200 elisa kit

OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13. 2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.

Product Categories/Family for CD200 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 311033489
• NCBI GeneID: 4345
• UniProt Accession #: P41217; Q6J2Q6; Q6PIQ4; Q8TB85; Q9H3J3; B3KQI1; B4DLW9; D3DN65
• Molecular Weight: 32,975 Da
• NCBI Official Full Name: OX-2 membrane glycoprotein
• NCBI Official Synonym Full Names: CD200 molecule
• NCBI Official Symbol: CD200
• NCBI Official Synonym Symbols: MRC; MOX1; MOX2; OX-2
• NCBI Protein Information: OX-2 membrane glycoprotein
• UniProt Protein Name: OX-2 membrane glycoprotein
• Protein Family: OX-2 membrane glycoprotein
• UniProt Gene Name: CD200
• UniProt Synonym Gene Names: MOX1; MOX2

NCBI Description

This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.