Testing Data
(Staining of equine peripheral blood lymphocytes with Rat anti Equine CD2, visualised with Rabbit anti Rat IgG:FITC (MBS235146))
Rat CD2 Monoclonal Antibody | anti-CD2 antibody
RAT ANTI HORSE CD2
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
CD2; Monoclonal Antibody; RAT ANTI HORSE CD2; anti-CD2 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
Mac288
Form/Format
Tissue Culture Supernatant - liquid
Sequence Length
347
Applicable Applications for anti-CD2 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 50ul of the suggested working dilution to label 7.5 x 105 cells in 100ul.
Flow Cytometry: Maximum Dilution: 1/200
Flow Cytometry: Maximum Dilution: 1/200
Perservative Stabilisers
0.1% Sodium Azide
Target Species
Horse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of equine peripheral blood lymphocytes with Rat anti Equine CD2, visualised with Rabbit anti Rat IgG:FITC (MBS235146))
Testing Data
(Staining of equine peripheral blood lymphocytes with Rat anti Equine CD2, visualised with Rabbit anti Rat IgG:FITC (MBS235146))
Related Product Information for anti-CD2 antibody
Rat anti Horse CD2 antibody, clone Mac288 recognizes equine CD2, a 347 amino acid, single pass type-1 transmembrane glycoprotein containing single Ig-like C2-type and Ig-like V-type domains. Equine CD2 is expressed by a large proportion of peripheral blood lymphocytes, most T-cells and few B-cells, it is not present on granulocytes. Immunohistochemical staining shows expression in most T cells of lymph node paracortex and occasional staining of lymphocytes in the follicular areas. In the thymus, rat anti horse CD2 antibody, clone Mac288 stains the majority of mature thymocytes in the medulla and at least half of the immature thymocytes in the cortex (Kydd et al. 1994). Rat anti Horse CD2 antibody, clone Mac288 recognizes CD2 in Haflinger (Üner et al. 2013) and Lusitano horse breeds (Ferreira-Dias et al. 2005). Rat anti Horse CD2 antibody, clone Mac288 is a reliable pan T-cell marker for analysis of cell mediated immunity in horses infected with equine herpes virus by multi-parameter flow cytometry (Platt et al. 2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,864 Da
NCBI Official Full Name
T-cell surface antigen CD2
NCBI Official Symbol
CD2
NCBI Protein Information
T-cell surface antigen CD2; T-lymphocyte surface antigen precursor CD2
UniProt Protein Name
T-cell surface antigen CD2
Protein Family
UniProt Gene Name
CD2
UniProt Entry Name
CD2_HORSE
Uniprot Description
CD2 interacts with lymphocyte function-associated antigen (LFA-3) to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
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Product Notes
The CD2 cd2 (Catalog #AAA214914) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD2 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS). Flow Cytometry: Use 50ul of the suggested working dilution to label 7.5 x 105 cells in 100ul. Flow Cytometry: Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD2 cd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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