WB (Western Blot)
(Detection of human Sequestosome-1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody AAA212049 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
Rabbit anti-Human Sequestosome-1 Polyclonal Antibody | anti-SQSTM1 antibody
Rabbit anti-Sequestosome-1 Antibody, Affinity Purified
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Sequestosome-1, Antibody; Rabbit anti-Sequestosome-1 Antibody, Affinity Purified; p60; ZIP3; ubiquitin-binding protein p62; sequestosome-1; phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; phosphotyrosine independent ligand for the Lck SH2 domain p62; PDB3; p62; oxidative stress induced like; OSIL; NADGP; FTDALS3; EBIAP; EBI3-associated protein p60; EBI3-associated protein of 60 kDa; DMRV; A170; p62B; Sequestosome-1; sequestosome 1; anti-SQSTM1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SQSTM1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Orangutan
Immunogen
Between 390 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Sequestosome-1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody AAA212049 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human Sequestosome-1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody AAA212049 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human Sequestosome-1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Sequestosome-1 (Cat. No. AAA212049 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate)
IHC (Immunohistochemistry)
(Detection of human Sequestosome-1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Sequestosome-1 (Cat. No. AAA212049 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate)
Related Product Information for anti-SQSTM1 antibody
Sequestosome-1 is an adapter protein that binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. Sequestosome-1 may play a role in titin/TTN downstream signaling in muscle cells, and may regulate signaling cascades through ubiquitination. Sequestosome-1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13501].
Product Categories/Family for anti-SQSTM1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,629 Da
NCBI Official Full Name
sequestosome-1 isoform 1
NCBI Official Synonym Full Names
sequestosome 1
NCBI Official Symbol
SQSTM1
NCBI Official Synonym Symbols
p60; p62; A170; OSIL; PDB3; ZIP3; p62B
NCBI Protein Information
sequestosome-1; EBIAP; EBI3-associated protein p60; oxidative stress induced like; ubiquitin-binding protein p62; EBI3-associated protein of 60 kDa; phosphotyrosine independent ligand for the Lck SH2 domain p62; phosphotyrosine-independent ligand for the
UniProt Protein Name
Sequestosome-1
UniProt Gene Name
SQSTM1
UniProt Synonym Gene Names
ORCA; OSIL; EBIAP; p60
UniProt Entry Name
SQSTM_HUMAN
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Product Notes
The SQSTM1 sqstm1 (Catalog #AAA212049) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Sequestosome-1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sequestosome-1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SQSTM1 sqstm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sequestosome-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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