Mouse anti-Human ASF1B Monoclonal Antibody | anti-ASF1B antibody

Anti-ASF1B Antibody, Mouse Monoclonal

Cat. Num. AAA8107332

• Gene Names: ASF1B; CIA-II
• Reactivity: Human
• Applications: Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: ASF1B; Monoclonal Antibody; Anti-ASF1B Antibody; Mouse Monoclonal; ASF1B Antibody; Mouse MAb; anti-silencing function 1B histone chaperone; Anti-CIA-II Antibody; anti-ASF1B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 02
• Specificity: Human ASF1B
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ASF1B antibody: Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ICC/IF: 1:20-1:100
• Immunogen: Recombinant Human ASF1B protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ASF1B (rh ASF1B). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of Human ASF1B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ASF1B monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to nucleus.)

Related Product Information for anti-ASF1B antibody

The histone chaperone anti-silencing factor 1a (ASF1a) interacts with MDC1 and is recruited to sites of DSBs to facilitate the interaction of phospho-ATM with MDC1 and phosphorylation of MDC1, which are required for the recruitment of RNF8/RNF168 histone ubiquitin ligases. Thus, ASF1a deficiency reduces histone ubiquitination at DSBs, decreasing the recruitment of 53BP1, and decreases NHEJ, rendering cells more sensitive to DSBs. This role of ASF1a in DSB repair cannot be provided by the closely related ASF1b and does not require its histone chaperone activity. Homozygous deletion of ASF1A is seen in 10%-15% of certain cancers, suggesting that loss of NHEJ may be selected in some malignancies and that the deletion can be used as a molecular biomarker for cancers susceptible to radiotherapy or to DSB-inducing chemotherapy. Anti-silencing function 1 (ASF1) is a histone H3-H4 chaperone involved in DNA replication and repair, and transcriptional regulation. Here, we identify ASF1B, the mammalian paralog to ASF1, as a proliferation-inducing histone chaperone in human beta-cells. Overexpression of ASF1B led to distinct transcriptional signatures consistent with increased cellular proliferation and reduced cellular death.

NCBI and Uniprot Product Information

• NCBI GI #: 8922549
• NCBI GeneID: 55723
• NCBI Accession #: NP_060624.1
• NCBI GenBank Nucleotide #: NM_018154.2
• UniProt Accession #: Q9NVP2; Q53G51; Q9NVZ0
• Molecular Weight: 22,434 Da
• NCBI Official Full Name: histone chaperone ASF1B
• NCBI Official Synonym Full Names: anti-silencing function 1B histone chaperone
• NCBI Official Symbol: ASF1B
• NCBI Official Synonym Symbols: CIA-II
• NCBI Protein Information: histone chaperone ASF1B; hAsf1; hAsf1b; hCIA-II; CCG1-interacting factor A-II; ASF1 anti-silencing function 1 homolog B; anti-silencing function protein 1 homolog B
• UniProt Protein Name: Histone chaperone ASF1B
• Protein Family: Histone chaperone
• UniProt Gene Name: ASF1B
• UniProt Synonym Gene Names: hAsf1; hAsf1b; CIA-II; hCIA-II
• UniProt Entry Name: ASF1B_HUMAN

NCBI Description

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008]

Uniprot Description

ASF1B: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis. Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with HAT1, NASP, TAF1, TLK1 and TLK2. Highly expressed in testis and at lower levels in colon, small intestine and thymus. Belongs to the ASF1 family.

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: nucleoplasm; protein complex; nuclear chromatin

Molecular Function: protein binding; histone binding

Biological Process: regulation of transcription, DNA-dependent; DNA replication-independent nucleosome assembly; transcription, DNA-dependent; multicellular organismal development; spermatogenesis; chromatin modification; cell differentiation; DNA replication-dependent nucleosome assembly