Mouse CD2 Monoclonal Antibody | anti-CD2 antibody

MOUSE ANTI HUMAN CD2

Cat. Num. AAA212050

• Gene Names: CD2; T11; SRBC; LFA-2
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CD2; Monoclonal Antibody; MOUSE ANTI HUMAN CD2; anti-CD2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: LT2
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1 mg/ml (varies by lot)
• Sequence Length: 351

• Applicable Applications for anti-CD2 antibody: Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Immunohistology - Frozen: Minimum Dilution: 1/200; Maximum Dilution: 1/400; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Immunogen: Normal human peripheral blood lymphocytes.; Histology Positive Control Tissue
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD2 (MBS212049))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD2:Biotin (MBS210313))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD2:RPE (MBS213559))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD2:Alexa Fluor 647 (MBS212049A647))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD2:FITC (MBS210628))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD2)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD2:Low Endotoxin (MBS219466))

Related Product Information for anti-CD2 antibody

Mouse anti Human CD2 antibody, clone LT2 recognizes human CD2, a single chain transmembrane glycoprotein of 50kD, expressed by T cells, NK cells and thymocytes. CD2 is a receptor for CD58 (LFA-3), and is important in mediating the interaction between T cells and target cells or antigen presenting cells. Mouse anti Human CD2 antibody, clone LT2 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation.

NCBI and Uniprot Product Information

• NCBI GI #: 156071472
• NCBI GeneID: 914
• NCBI Accession #: NP_001758.2
• NCBI GenBank Nucleotide #: NM_001767.3
• UniProt Accession #: P06729; Q96TE5
• Molecular Weight: 39,448 Da
• NCBI Official Full Name: T-cell surface antigen CD2
• NCBI Official Synonym Full Names: CD2 molecule
• NCBI Official Symbol: CD2
• NCBI Official Synonym Symbols: T11; SRBC; LFA-2
• NCBI Protein Information: T-cell surface antigen CD2; CD2 antigen (p50), sheep red blood cell receptor; LFA-3 receptor; T-cell surface antigen T11/Leu-5; erythrocyte receptor; lymphocyte-function antigen-2; rosette receptor
• UniProt Protein Name: T-cell surface antigen CD2
• Protein Family: CD2
• UniProt Gene Name: CD2
• UniProt Synonym Gene Names: SRBC
• UniProt Entry Name: CD2_HUMAN

NCBI Description

CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it.[supplied by OMIM, Jul 2010]

Uniprot Description

CD2: CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.

Protein type: Cell surface; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: internal side of plasma membrane; cell surface; anchored to plasma membrane; integral to plasma membrane; extracellular region; plasma membrane; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; receptor activity; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; cell surface receptor linked signal transduction; T cell activation; apoptosis; natural killer cell activation; regulation of T cell differentiation; blood coagulation; positive regulation of tumor necrosis factor production; lipid raft polarization; leukocyte migration; positive regulation of myeloid dendritic cell activation