Mouse anti-Human CD1b Monoclonal Antibody | anti-CD1B antibody

CD1b (T-Cell Surface Glycoprotein) Mouse Monoclonal Antibody

Cat. Num. AAA439494

• Gene Names: CD1B; R1; CD1; CD1A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD1b; Monoclonal Antibody; CD1b (T-Cell Surface Glycoprotein) Mouse Monoclonal Antibody; A1 domain; CD1B; Cortical thymocyte antigen CD1B; hTa1 thymocyte antigen; Ly38; T cell surface glycoprotein CD1b; T6/Leu6; anti-CD1B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone Number: 100-1A5
• Specificity: The mouse monoclonal antibody recognizes CD1b, a 44kDa type I glycoprotein associated with beta2-microglobulin (Workshop IV; Code T015). It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 333

• Applicable Applications for anti-CD1B antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Functional Studies (Order Ab without BSA & Azide)
• Cellular Localization: Cell Surface
• Immunogen: Stimulated human leukocytes
• Hu-Chromosome Location: 1q23.1
• Positive Control: MOLT4 cells. Human thymus.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Knapp W. et al. (eds) Leukocyte Typing IV, p251-263, Oxford University Press, Oxford, 1989. Battistini L, et al. CD1b is expressed in multiple sclerosis lesions. J Neuroimmunol 1996, 67(2):145-151. Khalili-Shirazi A, et al. The distribution of CD1 molecules in inflammatory neuropathy. J Neurol Sci 1998,158(2):154-163. Maher JK and Kronenberg M. The role of CD1 molecules in immune responses to infection. Curr Opin Immunol 1997, 9(4):456-461Blumberg RS et al. Structure and function of the CD1 family of MHC-like cell surface proteins. Immunol Rev 1995, 147:5-29. Salamone MC et al. Analysis of CD1 molecules on haematological malignancies of myeloid and lymphoid origin. II. Intracellular detection of CD1 antigens. Dis Markers 1990, 8(5):275-281

NCBI and Uniprot Product Information

• NCBI GI #: 4502645
• NCBI GeneID: 910
• NCBI Accession #: NP_001755.1
• NCBI GenBank Nucleotide #: NM_001764.2
• UniProt Accession #: P29016; Q5TDK9; Q5TDL0; Q9UMM2
• Molecular Weight: 44kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD1b
• NCBI Official Synonym Full Names: CD1b molecule
• NCBI Official Symbol: CD1B
• NCBI Official Synonym Symbols: R1; CD1; CD1A
• NCBI Protein Information: T-cell surface glycoprotein CD1b
• UniProt Protein Name: T-cell surface glycoprotein CD1b
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD1B
• UniProt Entry Name: CD1B_HUMAN

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens. [provided by RefSeq, Jul 2008]

Uniprot Description

CD1B: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cell surface; plasma membrane

Molecular Function: beta-2-microglobulin binding; endogenous lipid antigen binding; exogenous lipid antigen binding; protein binding

Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; regulation of immune response