Mouse anti-Human CD1b Monoclonal Antibody | anti-CD1b antibody

Anti-CD1b Antibody (RIV12)

Cat. Num. AAA847351

• Gene Names: CD1B; R1; CD1; CD1A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Protein A/G purification
• Synonyms: CD1b; Monoclonal Antibody; Anti-CD1b Antibody (RIV12); A1 domain; CD1B; Cortical thymocyte antigen CD1B; hTa1 thymocyte antigen; Ly38; T cell surface glycoprotein CD1b; T6/Leu6; anti-CD1b antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone Number: RIV12
• Specificity: Monoclonal Antibody to detect CD1b in human samples
• Purity/Purification: Protein A/G purification
• Form/Format: Colorless liquid. In 10mM PBS with 0.05% BSA & 0.05% azide

• Applicable Applications for anti-CD1b antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: FC/FACS: 0.5-1ug/1x106 cells in 0.1ml; IF: 0.5-1ug/ml
• Immunogen: Human peripheral lymphocytes
• Positive Control: IHC: Human PBMC's
• Preparation and Storage: Store at -20 degree C; The antibody solution should be gently mixed before use; Shelf life: ~12 months

Flow Cytometry (FC/FACS)

Related Product Information for anti-CD1b antibody

The mouse monoclonal antibody recognizes CD1b, a 44kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells.

Product Categories/Family for anti-CD1b antibody

Immunology; Antibodies & Supporting Tools; CD Antigens

NCBI and Uniprot Product Information

• NCBI GI #: 4502645
• NCBI GeneID: 910
• NCBI Accession #: NP_001755.1
• NCBI GenBank Nucleotide #: NM_001764.2
• UniProt Accession #: P29016; Q5TDK9; Q5TDL0; Q9UMM2
• Molecular Weight: 30,740 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD1b
• NCBI Official Synonym Full Names: CD1b molecule
• NCBI Official Symbol: CD1B
• NCBI Official Synonym Symbols: R1; CD1; CD1A
• NCBI Protein Information: T-cell surface glycoprotein CD1b; CD1B antigen, b polypeptide; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3
• UniProt Protein Name: T-cell surface glycoprotein CD1b
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD1B
• UniProt Entry Name: CD1B_HUMAN

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens. [provided by RefSeq, Jul 2008]

Uniprot Description

CD1B: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cell surface; lysosomal membrane; plasma membrane; integral to membrane; endosome membrane

Molecular Function: protein binding; beta-2-microglobulin binding; exogenous lipid antigen binding; endogenous lipid antigen binding

Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; immune response