Mouse anti-Human CD180 Monoclonal Antibody | anti-CD180 antibody

Anti-Hu CD180 APC

Cat. Num. AAA1800630

• Gene Names: CD180; LY64; Ly78; RP105
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD180; Monoclonal Antibody; Anti-Hu CD180 APC; CD180 molecule; LY64; Ly78; RP105; Bgp95; anti-CD180 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: G28-8
• Specificity: The mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
• Purity/Purification: The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
• Sequence Length: 661

• Applicable Applications for anti-CD180 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: QC tested
• Immunogen: Human tonsillar B cells
• Conjugation: APC
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD180 (G28-8) APC.)

Related Product Information for anti-CD180 antibody

CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.

NCBI and Uniprot Product Information

• NCBI GI #: 1843411
• NCBI GeneID: 4064
• UniProt Accession #: Q99467
• NCBI Official Full Name: RP105
• NCBI Official Synonym Full Names: CD180 molecule
• NCBI Official Symbol: CD180
• NCBI Official Synonym Symbols: LY64; Ly78; RP105
• NCBI Protein Information: CD180 antigen
• UniProt Protein Name: CD180 antigen
• Protein Family: CD180 antigen
• UniProt Gene Name: CD180
• UniProt Synonym Gene Names: LY64; RP105
• UniProt Entry Name: CD180_HUMAN

NCBI Description

CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq, Jul 2008]

Uniprot Description

CD180: May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: plasma membrane; integral to membrane

Biological Process: positive regulation of lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; B cell proliferation during immune response