CD180 recombinant protein

CD180 Recombinant Protein

Cat. Num. AAA3003513

• Gene Names: CD180; LY64; Ly78; RP105
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Synonyms: CD180; CD180 Recombinant Protein; CD180 recombinant protein

• Host: E Coli
• Purity/Purification: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Form/Format: PBS, 4M Urea, PH7.4
• Concentration: >=0.5mg/ml (varies by lot)
• Sequence: WDQMCIEKEANKTYNCENLGLSEIPDTLPNTTEFLEFSFNFLPTIHNRTFSRLMNLTFLDLTRCQINWIHEDTFQSHHQLSTLVLTGNPLIFMAETSLNGPKSLKHLFLIQTGISNLEFIPVHNLENLESLYLGSNHISSIKFPKDFPARNLKVLDFQNNAIHYISREDMRSLEQAINLSLNFNGNNVKGIELGAFDSTIFQSLNFGGTPNLSVIFNGLQNSTTQSLWLGTFEDIDDEDISSAMLKGLCEMSVESLNLQEHRFSDISSTTFQCFTQLQELDLTATHLKGLPSGMKGLNLLKKLVLSVNHFDQLCQISAANFPSLTHLYIRGNVKKLHLGVGCLEKLGNLQTLDLSHNDIEASDCCSLQLKNLSHLQTLNLSHNEPLGLQSQAFKECPQLELLDLAFTRLHINAPQSPFQNLHFLQVLNLTYCFLDTSNQHLLAGLPVLRHLNLKGNHFQDGTITKTNLLQTVGSLEVLILSSCGLLSIDQQAFHSLGKMSHVDLSHNSLTCDSIDSLSHLKGIYLNLAANSINIISPRLLPILSQQSTINLSHNPLDCTCSNIHFLTWYKENLHKLEGSEETTCANPPSLRGVKLSDVKLSCG

• Tag: His-tag in C-terminal
• Expression Vector: pet-22b(+)
• BP: 1821
• Restriction Site: NdeI-XhoI
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Testing Data

()

Related Product Information for CD180 recombinant protein

Background: CD180/RP105 is a type-I transmembrane glycoprotein that is restricted to expression in mature B-cells. Research studies have shown that CD180 engages signaling cascades in mature B-cells that drive cell proliferation. Indeed, CD180 functions with TLR4 as an LPS receptor on B-cells and, upon binding LPS, transmits proliferation and survival signals that require the Vav family of guanine nucleotide exchange factors.

NCBI and Uniprot Product Information

• NCBI GI #: 1843411
• NCBI GeneID: 4064
• UniProt Accession #: Q99467; Q32MM5; B2R7Z7
• Molecular Weight: 74,179 Da
• NCBI Official Full Name: RP105
• NCBI Official Synonym Full Names: CD180 molecule
• NCBI Official Symbol: CD180
• NCBI Official Synonym Symbols: LY64; Ly78; RP105
• NCBI Protein Information: CD180 antigen; lymphocyte antigen 64; radioprotective 105 kDa protein; lymphocyte antigen-64, radioprotective, 105kDa
• UniProt Protein Name: CD180 antigen
• Protein Family: CD180 antigen
• UniProt Gene Name: CD180
• UniProt Synonym Gene Names: LY64; RP105
• UniProt Entry Name: CD180_HUMAN

NCBI Description

CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq, Jul 2008]

Uniprot Description

CD180: May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: plasma membrane; integral to membrane

Biological Process: positive regulation of lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; B cell proliferation during immune response