RP105/CD180 Recombinant Protein | CD180 recombinant protein

RP105/CD180 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120801

• Gene Names: CD180; LY64; Ly78; RP105
• Purity: >92% as determined by SDS-PAGE
• Synonyms: RP105/CD180; RP105/CD180 Protein; Human; Recombinant (His Tag); Human CD180/RP105/LY64 Protein (His Tag); LY64 Protein; Ly78 Protein; RP105 Protein; CD180 molecule; CD180 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly626

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Trp 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CD180 (NP_005573.2) extracellular domain (Met 1-Gly 626) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD180 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD180, also known as RP105, is a B-cell surface molecule belonging to the family of pathogen receptors, Toll-like receptors (TLR). CD180 has an extracellular leucine-rich repeats and a short cytoplasmic tail. CD180/RP105 interacts with an extracellular molecule named MD1 and then together form the cell surface receptor complex RP105/MD1 which induces B-cell activation in humans and mice, leading to proliferation and up-regulation of a costimulatory molecule, B7.2/CD86. CD180/RP105 also has a role in LPS response because B cells lacking RP105 show hyporesponsiveness to LPS.

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Nagai Y, et al. (2002) Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Journal of the American society of hematology. 99(5): 1699-705.

NCBI and Uniprot Product Information

• NCBI GI #: 167555127
• NCBI GeneID: 4064
• NCBI Accession #: NP_005573.2
• NCBI GenBank Nucleotide #: NM_005582.2
• UniProt Accession #: Q99467; Q32MM5; B2R7Z7
• Molecular Weight: 74,179 Da
• NCBI Official Full Name: CD180 antigen
• NCBI Official Synonym Full Names: CD180 molecule
• NCBI Official Symbol: CD180
• NCBI Official Synonym Symbols: LY64; Ly78; RP105
• NCBI Protein Information: CD180 antigen; lymphocyte antigen 64; lymphocyte antigen-64, radioprotective, 105kDa; radioprotective 105 kDa protein
• UniProt Protein Name: CD180 antigen
• Protein Family: CD180 antigen
• UniProt Gene Name: CD180
• UniProt Synonym Gene Names: LY64; RP105
• UniProt Entry Name: CD180_HUMAN

Uniprot Description

CD180: May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: plasma membrane; integral to membrane

Biological Process: positive regulation of lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; B cell proliferation during immune response