Rabbit anti-Human RP105 Polyclonal Antibody | anti-CD180 antibody

RP105 Antibody

Cat. Num. AAA151419

• Gene Names: CD180; LY64; Ly78; RP105
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: RP105 Antibody is affinity chromatography purified via peptide column.
• Synonyms: RP105; Polyclonal Antibody; RP105 Antibody; LY64; Ly78; CD180 antigen; Lymphocyte antigen 64; CD180 molecule; anti-CD180 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: RP105 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 661

• Applicable Applications for anti-CD180 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: RP105 antibody can be used for the detection of RP105 by Western blot at 0.5 - 1 mug/mL. Despite its predicted molecular weight, RP105 often migrates at 95 - 105 kDa. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: RP105 antibody was raised against a 11 amino acid synthetic peptide from near the amino terminus of human RP105.
• Buffer: RP105 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: RP105 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RP105 in human spleen tissue lysate with RP105 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RP105 in human spleen tissue with RP105 antibody at 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of RP105 in human spleen tissue with RP105 antibody at 20 μg/mL.)

Related Product Information for anti-CD180 antibody

RP105 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The signaling of these TLRs is kept under tight control by the expression of endogenous inhibiting proteins. One such protein is RP105, a recently identified homolog to TLR4 that, with MD-1, interacts with and inhibits the TLR4/MD-2 signaling pathway. It has also been suggested that the RP105/MD-1 complex influences antibody production mediated by both TLR4/MD-2 and TLR2 receptor complexes.

NCBI and Uniprot Product Information

• NCBI GI #: 1843411
• NCBI GeneID: 4064
• UniProt Accession #: Q99467; Q32MM5; B2R7Z7
• Molecular Weight: 74,179 Da
• NCBI Official Full Name: RP105
• NCBI Official Synonym Full Names: CD180 molecule
• NCBI Official Symbol: CD180
• NCBI Official Synonym Symbols: LY64; Ly78; RP105
• NCBI Protein Information: CD180 antigen; lymphocyte antigen 64; radioprotective 105 kDa protein; lymphocyte antigen-64, radioprotective, 105kDa
• UniProt Protein Name: CD180 antigen
• Protein Family: CD180 antigen
• UniProt Gene Name: CD180
• UniProt Synonym Gene Names: LY64; RP105
• UniProt Entry Name: CD180_HUMAN

NCBI Description

CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq, Jul 2008]

Uniprot Description

CD180: May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: integral to membrane; plasma membrane

Biological Process: positive regulation of lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; B cell proliferation during immune response